A0A328L784 · A0A328L784_9BACI

  • Protein
    3-isopropylmalate dehydrogenase
  • Gene
    leuB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site94substrate
Binding site104substrate
Binding site130substrate
Site137Important for catalysis
Site183Important for catalysis
Binding site215Mg2+ (UniProtKB | ChEBI)
Binding site215substrate
Binding site239Mg2+ (UniProtKB | ChEBI)
Binding site243Mg2+ (UniProtKB | ChEBI)
Binding site273-285NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • ORF names
      B5P42_29310

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • SRB_331
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus

Accessions

  • Primary accession
    A0A328L784

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-340Isopropylmalate dehydrogenase-like

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    354
  • Mass (Da)
    38,586
  • Last updated
    2018-10-10 v1
  • Checksum
    8715CB7D2DFC3802
MEKRIVCLAGDGVGPEIMESAKEVLHMVERLYGHHFHLQDEYFGGAAIDLNGQPLPQRTLAACLASDAVLLGAVGGPRWDDAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKNADEIDFVVVRELTGGIYFSYPKERTEESATDTLTYHRHEIERIVSYAFQLASKREKKVTSIDKANVLESSKLWRAVTEEVALRYPNVELEHILVDAAAMELIRNPRRFDVIVTENLFGDILSDEASVLAGSLGMLPSASHAENGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGYAIEEAISAVLQSGKCTADIGGNETTTSFTRAVIQEMEEQALVGRGR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NADV01000064
EMBL· GenBank· DDBJ
RAN72416.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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