A0A327ZNC7 · A0A327ZNC7_9STAP

Function

function

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ cation per monomer.
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Note: Binds 1 nickel ion per monomer.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site98May play a role in metal incorporation in vivo
Binding site99Fe2+ (UniProtKB | ChEBI)
Binding site99Ni2+ (UniProtKB | ChEBI)
Binding site101Fe2+ (UniProtKB | ChEBI)
Binding site101Ni2+ (UniProtKB | ChEBI)
Site104May play a role in transmitting local conformational changes
Binding site105Fe2+ (UniProtKB | ChEBI)
Binding site105Ni2+ (UniProtKB | ChEBI)
Site107Important to generate the dianion
Binding site144Fe2+ (UniProtKB | ChEBI)
Binding site144Ni2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionacireductone dioxygenase (Ni2+-requiring) activity
Molecular Functionacireductone dioxygenase [iron(II)-requiring] activity
Molecular Functioniron ion binding
Molecular Functionnickel cation binding
Biological ProcessL-methionine salvage from methylthioadenosine
Biological ProcessL-methionine salvage from S-adenosylmethionine

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acireductone dioxygenase
  • Alternative names
    • 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
      (DHK-MTPene dioxygenase
      )
    • Acireductone dioxygenase (Fe(2+)-requiring)
      (ARD'
      ; Fe-ARD
      ) (EC:1.13.11.54
      ) . EC:1.13.11.54 (UniProtKB | ENZYME | Rhea)
    • Acireductone dioxygenase (Ni(2+)-requiring)
      (ARD
      ; Ni-ARD
      ) (EC:1.13.11.53
      ) . EC:1.13.11.53 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      mtnD
    • ORF names
      BHU61_11740

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 01/688
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Staphylococcaceae > Macrococcus

Accessions

  • Primary accession
    A0A327ZNC7

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Sequence similarities

Belongs to the acireductone dioxygenase (ARD) family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    172
  • Mass (Da)
    19,937
  • Last updated
    2018-10-10 v1
  • Checksum
    6868C3143AFCB8B9
MAYLIFQETGKRLDNQEEIHAFLESNEVIYEQWDTSKLPDTLKENYNLTDEDKSEILNTFKEEIDAISARRGYKASDVISLSDTTPNLDELLTNFQREHHHTDDEVRFIVSGNGVFVIQGKDDQFFEVHLDPGDLISVPPNVRHYFTLSDNRKVVAVRIFVDEAGWVPVYEK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PZJH01000008
EMBL· GenBank· DDBJ
RAK43839.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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