A0A317CJL8 · A0A317CJL8_9GAMM
- ProteinPhosphoribosylformylglycinamidine synthase
- GenepurL
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1297 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.
Catalytic activity
- N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N1-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H+
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 307-318 | ATP (UniProtKB | ChEBI) | |||
Binding site | 678 | ATP (UniProtKB | ChEBI) | |||
Binding site | 679 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 718 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 722 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 886 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 888 | ATP (UniProtKB | ChEBI) | |||
Active site | 1137 | Nucleophile | |||
Active site | 1262 | ||||
Active site | 1264 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoribosylformylglycinamidine synthase activity | |
Biological Process | 'de novo' IMP biosynthetic process | |
Biological Process | glutamine metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoribosylformylglycinamidine synthase
- EC number
- Short namesFGAM synthase ; FGAMS
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Thiotrichales > Thiotrichaceae > Leucothrix
Accessions
- Primary accessionA0A317CJL8
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 36-150 | Phosphoribosylformylglycinamidine synthase N-terminal | |||
Domain | 171-220 | Phosphoribosylformylglycinamidine synthase linker | |||
Region | 305-327 | Disordered | |||
Domain | 432-586 | PurM-like C-terminal | |||
Domain | 648-806 | FGAR-AT PurM-N-like | |||
Domain | 859-967 | PurM-like C-terminal | |||
Sequence similarities
In the N-terminal section; belongs to the FGAMS family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,297
- Mass (Da)140,494
- Last updated2018-10-10 v1
- MD5 ChecksumE1FA0EC56AB3F52BDEDC2CC49A9E53EA
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QGKL01000010 EMBL· GenBank· DDBJ | PWQ98748.1 EMBL· GenBank· DDBJ | Genomic DNA |