A0A2Z6GET8 · A0A2Z6GET8_9PROT

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

1824100200300400500600700800
TypeIDPosition(s)Description
Site33Interaction with DNA
Site143Interaction with DNA
Site144Interaction with DNA
Site147Interaction with DNA
Site159Interaction with DNA
Active site306O-(5'-phospho-DNA)-tyrosine intermediate
Site308Interaction with DNA
Site501Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      OYT1_ch2599

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • OYT1
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Nitrosomonadales > Gallionellaceae > Ferriphaselus

Accessions

  • Primary accession
    A0A2Z6GET8

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain3-117Toprim
Region167-172Interaction with DNA

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    824
  • Mass (Da)
    91,693
  • Last updated
    2018-12-05 v1
  • Checksum
    BDAA393B125862B7
MASNLLVVESPAKAKTLKKYLGKDFEVLASYGHVRDLIPKTGAIDVDNGYAMQYETIARNAKHVDAIAKAAAEADVVYLAPDPDREGEAIAWHLAELLRAKRGLKNKPLKRVVFHEITQSAVKEAVANPREILLPLVNAQQARRALDYLVGFNLSPLLWRKIRPGLSAGRVQSPALRLIVERELEIEAFRPQEYWTVHLDSQKNTLPFTAKLFQYHGKKVEQLTIGSEAEYRKIFDSLSDAKLPPKVVKVERRGKQRYAAAPFTTSTLQQEAVRKLGMTSERTMRLAQQLYEGIDIGGGTVGLITYMRTDSVALANEAVAEIRGYIGDNFAPDYLPKTSPVYKSKTKNAQEAHEAIRPTGIARTPDSVREFLTVDQARLYEMIWKRTLASQMTPARFDTVSVDIRLGGDTTLFRASGQTLVFPGFIAVYQEDTDDSTEEDGGKLPPLEEGDVLPQDKLYGEQHFTQPPPRFSEASLVKSLEEYGIGRPSTYASIISTLQAREYVVLEKKRFQPTDVGRVVNRFLTDHFTRYVDYGFTAQMEDELDEISEGKRDWIPVLDEFWKGFHQLIGEKKDVDRPGTEILEEACPKCGKPLSKRLGKRGSFIGCTGYPECDFTRNLSGEVESAEDARKELGTNEEGTLILLLRGPYGPYVQLGEAKEGDKKKPKRVSWPKELPLEAADLGMALKLLALPREVGLHPESGKKVVVNIGRFGPYIGHDGAFKSIPKTDSIFTIELARAVELLAQKKDNAATILRDLGVHPDDQKPIEVCDGRYGPYVRHGKVNATLPKDVTVDELTLSQALELLAAKAAKGKPAKKPARKAKA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP018738
EMBL· GenBank· DDBJ
BBE52111.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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