A0A2Z5WK09 · A0A2Z5WK09_9CAUD

  • Protein
    Terminase, large subunit
  • Gene
    17_2
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer once the capsid is full (headful packaging). Once the capsid is packaged with the DNA, the terminase complex is substituted by the tail.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: ATPase activity requires 1 Mg2+ ion per subunit. Nuclease activity probably requires 2 Mg2+ ions per subunit.

Activity regulation

Stimulated up to 50 to 100-fold by the terminase small subunit.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site33ATP (UniProtKB | ChEBI)
Binding site38ATP (UniProtKB | ChEBI)
Binding site97ATP (UniProtKB | ChEBI)
Active site151For ATPase activity
Binding site296Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site296Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Site304Modulates nuclease activity
Binding site353Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site437Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity

GO annotations

AspectTerm
Cellular Componentviral terminase, large subunit
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Biological Processchromosome organization
Biological Processviral DNA genome packaging

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Terminase, large subunit
  • Alternative names
    • DNA-packaging protein

Including 2 domains:

  • Recommended name
    ATPase
  • EC number
  • Recommended name
    Endonuclease
  • EC number

Gene names

    • Name
      17_2

Organism names

  • Taxonomic identifier
  • Organism
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus > Tequatrovirus pp01

Accessions

  • Primary accession
    A0A2Z5WK09

Proteomes

Subcellular Location

Interaction

Subunit

Interacts with the terminase small subunit; the active complex is composed of a pentamer of terminase large subunits and a dodecamer of terminase small subunits. Interacts with the portal protein.

Family & Domains

Features

Showing features for region, motif, domain.

TypeIDPosition(s)Description
Region26-196ATPase activity
Motif56-62Walker A motif
Motif146-151Walker B motif
Motif180-182ATPase coupling
Region255-454Nuclease activity
Domain294-467Terminase large subunit gp17-like C-terminal

Domain

The N-terminus contains an ATPase domain. The C-terminus contains an endonuclease domain.

Sequence similarities

Belongs to the Tequatrovirus large terminase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    505
  • Mass (Da)
    57,145
  • Last updated
    2018-10-10 v1
  • Checksum
    DCF7E48B1AA9F96F
MVEEWKKCRDDIVYFAETYCAITHIDYGVIKVQLRDYQRDMLKIMSSKRMTVCNLSRQLGKTTVVAIFLAHFVCFNKDKAVGILAHKGSMSAEVLDRTKQAIELLPDFLQPGIVEWNKGSIELDNGSSIGAYASSPDAVRGNSFAMIYIDECAFIPNFHDSWLAIQPVISSGRRSKIIITTTPNGLNHFYDIWTAAVEGKSGFEPYTAIWNSVKERLYNDEDIFDDGWQWSIQTINGSTLAQFRQEHTAAFEGTSGTLISGMKLAVMDFIEVTPDDHGFHRFKSPEPDRKYIATLDCSEGRGQDYHALHIIDVTDDVWEQVGVLHSNTISHLILPDIVMRYLVEYNECPVYIELNSTGVSVAKSLYMDLEYEGVICDSYTDLGMKQTKRTKAVGCSTLKDLIEKDKLIIHHRATIQEFRTFSEKGVSWAAEEGYHDDLVMSLVIFGWLSTQSKFIDYADKDDMRLASEVFSKELQDMSDDYAPVIFVDSVHSAEYVPVSHGMSMV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LC348379
EMBL· GenBank· DDBJ
BBC14503.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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