A0A2Z5UBX1 · A0A2Z5UBX1_9SPHN
- ProteinMultifunctional fusion protein
- GenecysN
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids633 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
APS kinase catalyzes the synthesis of activated sulfate.
Catalyzes the synthesis of activated sulfate.
Proposed to provide activated sulfate for transfer to Nod factor. ATP sulfurylase may be the GTPase, regulating ATP sulfurylase activity.
With CysD forms the ATP sulfurylase (ATPS) that catalyzes the adenylation of sulfate producing adenosine 5'-phosphosulfate (APS) and diphosphate, the first enzymatic step in sulfur assimilation pathway. APS synthesis involves the formation of a high-energy phosphoric-sulfuric acid anhydride bond driven by GTP hydrolysis by CysN coupled to ATP hydrolysis by CysD.
Catalytic activity
- sulfate + ATP + H+ = adenosine 5'-phosphosulfate + diphosphate
Pathway
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3.
Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 2/3.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 35-42 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GSVDDGKS | ||||||
Binding site | 114-118 | GTP (UniProtKB | ChEBI) | ||||
Sequence: DTPGH | ||||||
Binding site | 169-172 | GTP (UniProtKB | ChEBI) | ||||
Sequence: NKMD | ||||||
Binding site | 472-479 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GLSGSGKS | ||||||
Active site | 546 | Phosphoserine intermediate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | adenylylsulfate kinase activity | |
Molecular Function | ATP binding | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | guanosine tetraphosphate binding | |
Molecular Function | sulfate adenylyltransferase (ATP) activity | |
Biological Process | hydrogen sulfide biosynthetic process | |
Biological Process | phosphorylation | |
Biological Process | sulfate assimilation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameSulfate adenylyltransferase subunit 1
- EC number
- Alternative names
- Recommended nameAdenylyl-sulfate kinase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Sphingomonadales > Sphingomonadaceae > Sphingopyxis
Accessions
- Primary accessionA0A2Z5UBX1
Proteomes
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Heterodimer composed of CysD, the smaller subunit, and CysN.
Sulfate-activating enzymes, NodP and NodQ, may be physically associated.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 26-240 | Tr-type G | ||||
Sequence: KSLLRFITCGSVDDGKSTLIGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRFFTTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSFLAHLIGIKHIVLAVNKMDLVGYDKTVFERILLSYRAFASEIGITNFTAIPISGFKGDNITALSDNMPWFKGPALIEHLENVEIGAA |
Sequence similarities
Belongs to the APS kinase family.
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.
In the C-terminal section; belongs to the APS kinase family.
In the N-terminal section; belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length633
- Mass (Da)69,676
- Last updated2018-10-10 v1
- ChecksumB387D45037FF7654