A0A2Z5QKZ7 · ORS_RHODA

Function

function

Involved in the biosynthesis of acetate-derived aromatic tetraketides natural products, precursors of daurichromenic acid, an anti-human immunodeficiency viruses (HIV) meroterpenoid consisting of sesquiterpene and orsellinic acid (OSA) moieties (PubMed:27729920).
Accepts acetyl-CoA as starter substrate and produces orcinol as the major reaction product, along with four minor products including OSA, tetraacetate lactone, triacetate lactone and 2-acetylphloroglucinol (PubMed:27729920).

Catalytic activity

  • acetyl-CoA + 3 H+ + 3 malonyl-CoA = 4 CO2 + 4 CoA + orcinol
    This reaction proceeds in the forward direction.
  • acetyl-CoA + 2 H+ + 3 malonyl-CoA = 3 CO2 + 4 CoA + orsellinate
    This reaction proceeds in the forward direction.
  • acetyl-CoA + 3 H+ + 3 malonyl-CoA = 3 CO2 + 4 CoA + tetraacetate lactone
    This reaction proceeds in the forward direction.
  • acetyl-CoA + 2 H+ + 2 malonyl-CoA = 2 CO2 + 3 CoA + triacetate lactone
    This reaction proceeds in the forward direction.
  • acetyl-CoA + 3 H+ + 3 malonyl-CoA = 2-acetylphloroglucinol + 3 CO2 + 4 CoA
    This reaction proceeds in the forward direction.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
10.6 μMtetraacetic acid lactone (with acetyl-CoA as cosubstrate)
11.7 μMtriacetic acid lactone (with acetyl-CoA as cosubstrate)
9.98 μMorcinol (with acetyl-CoA as cosubstrate)
9.88 μMorsellinic acid (with acetyl-CoA as cosubstrate)
10.8 μMphloroacetophenone (with acetyl-CoA as cosubstrate)
17.1 μM4-hydroxy-6-propyl-2-pyrone (with butyryl-CoA as cosubstrate)
26.4 μM4-hydroxy-6-pentyl-2-pyrone (with hexanoyl-CoA as cosubstrate)
kcat is 0.0573 min-1 with tetraacetic acid lactone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.243 min-1 with triacetic acid lactone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 1.46 min-1 with orcinol as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.0675 min-1 with orsellinic acid as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 8.81x10-3 min-1 with phloroacetophenone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.405 min-1 with 4-hydroxy-6-propyl-2-pyrone as substrate (with butyryl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.289 min-1 with 4-hydroxy-6-pentyl-2-pyrone as substrate (with hexanoyl-CoA as cosubstrate) (PubMed:27729920).

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site164
Active site322
Active site355

GO annotations

AspectTerm
Molecular Functionacyltransferase activity, transferring groups other than amino-acyl groups
Molecular Functionidentical protein binding
Molecular Functionprotein homodimerization activity
Biological Processorcinol biosynthetic process
Biological Processpolyketide biosynthetic process
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Orcinol synthase
  • EC number
  • Short names
    RdORS

Gene names

    • Name
      ORS

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > Ericales > Ericaceae > Ericoideae > Rhodoreae > Rhododendron

Accessions

  • Primary accession
    A0A2Z5QKZ7

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004589881-412Orcinol synthase

Expression

Tissue specificity

Mainly expressed in young leaves, and barely in mature leaves and twigs.

Interaction

Subunit

Homodimer.

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    412
  • Mass (Da)
    45,708
  • Last updated
    2018-10-10 v1
  • Checksum
    3255B58013509D82
MALVNHRENVKGRAQILAIGTANPKNCFRQVDYPDYYFRVTKSDHLIDLKAKFKRMCEKSMIEKRYMHVNEEILEQNPSMNHGGEKMVSSLDVRLDMEIMEIPKLAAEAATKAMDEWGQPKSRITHLVFHSTLGTVMPGVDYELIKLLGLNPSVKRFMLYHLGCYGGGTVLRLAKDLAENNPGSRVLVLCCEMMPSGFHGPPSLQHAHLDILTGHAIFGDGAGAVIVGCVDPSGGTNGVVERGVRRYEQPLFEIHSAYQTVLPDSKDAVGGRLREAGLIYYLSKRLSNDVSGKIDECCLAEAFSAAIKDNFEDWNSLFWIVHPAGRPILDKLDAKLGLNKEKLRASRNVLRDYGNMWSSSVLFVLDEMRKGSIAQRKTTTGEGFEWGVLLGFGPGVTVETVVLRSVPTAKLK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
LC133082
EMBL· GenBank· DDBJ
BAV83003.1
EMBL· GenBank· DDBJ
mRNA

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