A0A2Z5QKZ7 · ORS_RHODA
- ProteinOrcinol synthase
- GeneORS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids412 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the biosynthesis of acetate-derived aromatic tetraketides natural products, precursors of daurichromenic acid, an anti-human immunodeficiency viruses (HIV) meroterpenoid consisting of sesquiterpene and orsellinic acid (OSA) moieties (PubMed:27729920).
Accepts acetyl-CoA as starter substrate and produces orcinol as the major reaction product, along with four minor products including OSA, tetraacetate lactone, triacetate lactone and 2-acetylphloroglucinol (PubMed:27729920).
Accepts acetyl-CoA as starter substrate and produces orcinol as the major reaction product, along with four minor products including OSA, tetraacetate lactone, triacetate lactone and 2-acetylphloroglucinol (PubMed:27729920).
Catalytic activity
- acetyl-CoA + 3 H+ + 3 malonyl-CoA = 4 CO2 + 4 CoA + orcinolThis reaction proceeds in the forward direction.
- acetyl-CoA + 2 H+ + 3 malonyl-CoA = 3 CO2 + 4 CoA + orsellinateThis reaction proceeds in the forward direction.
- acetyl-CoA + 3 H+ + 3 malonyl-CoA = 3 CO2 + 4 CoA + tetraacetate lactoneThis reaction proceeds in the forward direction.
- acetyl-CoA + 2 H+ + 2 malonyl-CoA = 2 CO2 + 3 CoA + triacetate lactoneThis reaction proceeds in the forward direction.
- acetyl-CoA + 3 H+ + 3 malonyl-CoA = 2-acetylphloroglucinol + 3 CO2 + 4 CoAThis reaction proceeds in the forward direction.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
10.6 μM | tetraacetic acid lactone (with acetyl-CoA as cosubstrate) | |||||
11.7 μM | triacetic acid lactone (with acetyl-CoA as cosubstrate) | |||||
9.98 μM | orcinol (with acetyl-CoA as cosubstrate) | |||||
9.88 μM | orsellinic acid (with acetyl-CoA as cosubstrate) | |||||
10.8 μM | phloroacetophenone (with acetyl-CoA as cosubstrate) | |||||
17.1 μM | 4-hydroxy-6-propyl-2-pyrone (with butyryl-CoA as cosubstrate) | |||||
26.4 μM | 4-hydroxy-6-pentyl-2-pyrone (with hexanoyl-CoA as cosubstrate) |
kcat is 0.0573 min-1 with tetraacetic acid lactone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.243 min-1 with triacetic acid lactone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 1.46 min-1 with orcinol as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.0675 min-1 with orsellinic acid as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 8.81x10-3 min-1 with phloroacetophenone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.405 min-1 with 4-hydroxy-6-propyl-2-pyrone as substrate (with butyryl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.289 min-1 with 4-hydroxy-6-pentyl-2-pyrone as substrate (with hexanoyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.243 min-1 with triacetic acid lactone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 1.46 min-1 with orcinol as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.0675 min-1 with orsellinic acid as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 8.81x10-3 min-1 with phloroacetophenone as substrate (with acetyl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.405 min-1 with 4-hydroxy-6-propyl-2-pyrone as substrate (with butyryl-CoA as cosubstrate) (PubMed:27729920).
kcat is 0.289 min-1 with 4-hydroxy-6-pentyl-2-pyrone as substrate (with hexanoyl-CoA as cosubstrate) (PubMed:27729920).
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 164 | |||||
Sequence: C | ||||||
Active site | 322 | |||||
Sequence: H | ||||||
Active site | 355 | |||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acyltransferase activity, transferring groups other than amino-acyl groups | |
Molecular Function | identical protein binding | |
Molecular Function | protein homodimerization activity | |
Biological Process | orcinol biosynthetic process | |
Biological Process | polyketide biosynthetic process | |
Biological Process | terpenoid biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOrcinol synthase
- EC number
- Short namesRdORS
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > Ericales > Ericaceae > Ericoideae > Rhodoreae > Rhododendron
Accessions
- Primary accessionA0A2Z5QKZ7
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000458988 | 1-412 | Orcinol synthase | |||
Sequence: MALVNHRENVKGRAQILAIGTANPKNCFRQVDYPDYYFRVTKSDHLIDLKAKFKRMCEKSMIEKRYMHVNEEILEQNPSMNHGGEKMVSSLDVRLDMEIMEIPKLAAEAATKAMDEWGQPKSRITHLVFHSTLGTVMPGVDYELIKLLGLNPSVKRFMLYHLGCYGGGTVLRLAKDLAENNPGSRVLVLCCEMMPSGFHGPPSLQHAHLDILTGHAIFGDGAGAVIVGCVDPSGGTNGVVERGVRRYEQPLFEIHSAYQTVLPDSKDAVGGRLREAGLIYYLSKRLSNDVSGKIDECCLAEAFSAAIKDNFEDWNSLFWIVHPAGRPILDKLDAKLGLNKEKLRASRNVLRDYGNMWSSSVLFVLDEMRKGSIAQRKTTTGEGFEWGVLLGFGPGVTVETVVLRSVPTAKLK |
Expression
Tissue specificity
Mainly expressed in young leaves, and barely in mature leaves and twigs.
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length412
- Mass (Da)45,708
- Last updated2018-10-10 v1
- Checksum3255B58013509D82