A0A2Z4I2Y0 · A0A2Z4I2Y0_9VIRU
- ProteinNon-structural protein 1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids667 (go to sequence)
- Protein existencePredicted
- Annotation score2/5
Function
function
Multifunctional protein which displays endonuclease and helicase activities required for initiating and directing viral DNA replication. Also plays a role in viral packaging and transactivation of several promoters. Binds site-specifically to 2-3 approximate tandem copies within the origins of replication (Ori), unwinds this hairpin region and nicks one DNA strand thereby initiating the rolling circle replication (RCR). Becomes covalently attached to the 5' end of the nick and provides a 3'OH for priming DNA synthesis. The helicase activity unwinds DNA in a 3'-5' direction on the longer strand. Participates in the transcriptional regulation of several promoters.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 228 | For nuclease activity | ||||
Sequence: Y |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | host cell nucleus | |
Molecular Function | ATP binding | |
Molecular Function | DNA binding | |
Molecular Function | endonuclease activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA replication | |
Biological Process | viral genome replication |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameNon-structural protein 1
Organism names
- Organism
- Strain
- Taxonomic lineageViruses > Monodnaviria > Shotokuvirae > Cossaviricota > Quintoviricetes > Piccovirales > Parvoviridae > Parvovirinae > Bocaparvovirus
Accessions
- Primary accessionA0A2Z4I2Y0
Subcellular Location
PTM/Processing
Keywords
- PTM
Interaction
Subunit
Homooligomer; when bound to DNA.
Structure
Family & Domains
Features
Showing features for domain, motif, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 24-287 | PV NS1-Nuc | ||||
Sequence: FKSPCYTYVIHLDLQDWRPHERALMDIYSKDVSVIIDDIVDTEKEFLLSDVNYEWKASLAMKLQNHHGLGQTLCESADIAVRRLFQLKQQNHNPQYSCFTQAEIGPSKIHVHVVCGGDGLTRFNAKKCTWIIAKFFWNHMLELWEHFESCYRPTNAQMAIGVQLRMLCERIKSKQDTTVTILTYKDRHNEQHAQRVDGPSYITNYLLPKNRQLVTWLDMDVCTPSVAWFETEKTYMCSHIDRQPITHYLRKGLHDRLVMNSSEE | ||||||
Motif | 133-135 | RCR-2 | ||||
Sequence: HVH | ||||||
Motif | 228-232 | RCR-3 | ||||
Sequence: YLLPK | ||||||
Domain | 402-569 | SF3 helicase | ||||
Sequence: NKVVRLLNIQGYNPIQVGHWVATVLSKKAGKQNTLCFYGPASTGKTNLAKAIVSAVKVYGCVNHLNKNFVFNDCQSKLIAWWEECVMHNDWVEPAKCLMGGTTFRVDRKHKDSAEQPHTPLIISTNHDIYTVVGGNTVTTVHEKPIRDRVVQFNFMKTLPQNFGEISV | ||||||
Region | 638-667 | Disordered | ||||
Sequence: DGDWTESADPGRTTRMRERETPSGGDGIIS | ||||||
Compositional bias | 645-659 | Basic and acidic residues | ||||
Sequence: ADPGRTTRMRERETP |
Family and domain databases
Sequence
- Sequence statusComplete
- Length667
- Mass (Da)76,254
- Last updated2018-10-10 v1
- ChecksumC49FA6177413F974
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 645-659 | Basic and acidic residues | ||||
Sequence: ADPGRTTRMRERETP |