Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

A0A2Z3N6R8 · A0A2Z3N6R8_GEOTH

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site46L-glutamine (UniProtKB | ChEBI)
Binding site213L-glutamine (UniProtKB | ChEBI)
Binding site215L-glutamine (UniProtKB | ChEBI)
Active site240Nucleophile
Binding site241L-glutamine (UniProtKB | ChEBI)
Binding site244L-glutamine (UniProtKB | ChEBI)
Binding site282L-glutamine (UniProtKB | ChEBI)
Binding site285L-glutamine (UniProtKB | ChEBI)
Active site325
Active site327

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      C1N76_03070

Organism names

Accessions

  • Primary accession
    A0A2Z3N6R8

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-164CPSase

Sequence similarities

Belongs to the CarA family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    354
  • Mass (Da)
    39,009
  • Last updated
    2018-10-10 v1
  • MD5 Checksum
    029F4696C5221755AAA298A032541B9D
MKAYLHVASGKTFSGELAAPLEEKVSGEIVFFTGMTGYQEVLTDPSYKNQIIVFTYPLIGNYGINEDDFESKRPHVEAVVVYEASGTGFHYGAKYSLAEYLQHWNIPLLTHVDTRALVKEIRTAGTMMAELSLSPIPAVADEEAAFPVRAVSTKTIETYGEAGPHLVLIDFGYKKSILQSLLARGCRVTVVPHDTAPEAIDALKPDGLVLSNGPGDPKQLSHQLPAIRQLIDRYPTLAICLGHQLVALAYGADTEKLRFGHRGANQPVWDAGKQKVMMTSQNHSYVVKEESLAGKPFDIRFINVNDGSVEGMVHRHKPILSVQYHPEAHPGPQDTGYIFDEFLQTVFKGENVYA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP027303
EMBL· GenBank· DDBJ
AWO73654.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help