A0A2V6U121 · A0A2V6U121_9BACT
- ProteinMultifunctional fusion protein
- GeneglyA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids559 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.
Negatively regulates transcription of bacterial ribonucleotide reductase nrd genes and operons by binding to NrdR-boxes.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
Cofactor
Pathway
Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.
Features
Showing features for binding site, site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | cobalt ion binding | |
Molecular Function | DNA binding | |
Molecular Function | glycine hydroxymethyltransferase activity | |
Molecular Function | methyltransferase activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | serine binding | |
Molecular Function | zinc ion binding | |
Biological Process | folic acid metabolic process | |
Biological Process | glycine biosynthetic process from serine | |
Biological Process | L-serine catabolic process | |
Biological Process | methylation | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | tetrahydrofolate interconversion |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameSerine hydroxymethyltransferase
- EC number
- Short namesSHMT ; Serine methylase
- Recommended nameTranscriptional repressor NrdR
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Candidatus Rokuibacteriota
Accessions
- Primary accessionA0A2V6U121
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 226 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 442-532 | ATP-cone | ||||
Sequence: PHVVKRDGRREPFDRQKLRGSILKSCEKRSVGVQAVDDVVADIEARLHERAEKEITSLEMGEIVMEHLQKLDQVAYVRFASVYRQFKDISQ |
Sequence similarities
Belongs to the NrdR family.
Belongs to the SHMT family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length559
- Mass (Da)61,205
- Last updated2018-09-12 v1
- Checksum9BC9CCE353FA8502