A0A2U8QQB5 · A0A2U8QQB5_9CAUD

Function

function

Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Coats the lagging-strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and DnaB-like SF4 replicative helicase, probably via its interaction with the helicase assembly factor. Together with DnaB-like SF4 replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange. Promotes also the formation of joint molecules. mRNA specific autogenous translational repressor.

Features

Showing features for binding site.

130220406080100120140160180200220240260280300
TypeIDPosition(s)Description
Binding site65Zn2+ (UniProtKB | ChEBI)
Binding site78Zn2+ (UniProtKB | ChEBI)
Binding site88Zn2+ (UniProtKB | ChEBI)
Binding site91Zn2+ (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular Functionmetal ion binding
Molecular Functionsingle-stranded DNA binding
Biological Processbidirectional double-stranded viral DNA replication
Biological ProcessDNA recombination
Biological ProcessDNA repair

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Single-stranded DNA-binding protein
  • Short names
    SSB protein
  • Alternative names
    • Gp32
    • Helix-destabilizing protein

Gene names

    • Name
      32
    • Synonyms
      ssb
    • ORF names
      vBEcoMNBG2_229

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Tequatrovirus > Tequatrovirus ecomnbg2

Accessions

  • Primary accession
    A0A2U8QQB5

Proteomes

Interaction

Subunit

Homodimer in the absence of DNA, monomer when binding DNA. Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the DnaB-like SF4 replicative helicase and the helicase assembly factor. Interacts (via C-terminus) with the viral SF1 dDA helicase. Interacts with the viral SF2 UvsW repair helicase.

Family & Domains

Features

Showing features for region, domain, compositional bias.

TypeIDPosition(s)Description
Region3-7LAST
Domain40-240Bacteriophage T4 Gp32 single-stranded DNA-binding
Region275-302Disordered
Compositional bias278-293Polar residues

Domain

The acidic C-terminus is involved in modulating the ssDNA binding properties. The N-terminus LAST motif is involved in the cooperative binding of the protein to ssDNA.

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    302
  • Mass (Da)
    33,522
  • Last updated
    2018-09-12 v1
  • Checksum
    032B78CB2FFF415D
MFKRKSTAELAAQMAKLAGNKGGFSSEDKGEWKLKLDNAGNGQAVIRFLPSKNDEQAPFAILVNHGFKKNGKWYIENCSSTHGDYDSCPVCQYISKNDLYNTDNKEYSLVKRKTSYWANILVVKDPAAPENEGKVFKYRFGKKIWDKINAMIAVDVEMGETPVDVTCPWEGANFVLKVKQVSGFSNYDESKFLNQSAIPNIDDESFQKELFEQMVDLSEMTSKDKFKSFEELSTKFSQVMGTAAMGGAAATAAKKADKVADDLDAFNVDDFKTKTEDDFMSSSSGSSSSADDTDLDDLLNDL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias278-293Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MH243439
EMBL· GenBank· DDBJ
AWM12009.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp