A0A2U8QP26 · A0A2U8QP26_9CAUD

Function

function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site114Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site116Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site222Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site327Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site327Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site411Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site411Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site412Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site414-416substrate
Binding site482substrate
Binding site560substrate
Site621Optimization of metal coordination by the polymerase active site
Binding site623Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site623Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Site706Optimization of metal coordination by the polymerase active site
Site714Essential for viral replication

GO annotations

AspectTerm
Molecular Function3'-5' exonuclease activity
Molecular FunctionDNA binding
Molecular FunctionDNA-directed DNA polymerase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding
Biological Processbidirectional double-stranded viral DNA replication
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA-directed DNA polymerase
  • EC number

Gene names

    • ORF names
      vBEcoMNBG1_050

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Straboviridae > Tevenvirinae > Mosigvirus > Mosigvirus mar005p1

Accessions

  • Primary accession
    A0A2U8QP26

Proteomes

Interaction

Subunit

Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, and the primase/helicase. Interacts with the polymerase clamp; this interaction constitutes the polymerase holoenzyme.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain106-293DNA-directed DNA polymerase family B exonuclease
Region248-264Beta hairpin
Domain368-494DNA-directed DNA polymerase family B multifunctional
Region380-903Polymerase
Domain555-629DNA-directed DNA polymerase family B multifunctional
Region705-708Binding of DNA in B-conformation
Region897-903Interaction with the polymerase clamp

Domain

The N-terminus contains the 3'-5' exonuclease activity. The C-terminus contains the polymerase activity and is involved in binding to the polymerase clamp protein. A beta hairpin structure is necessary for the proofreading function of the polymerase.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    903
  • Mass (Da)
    104,631
  • Last updated
    2018-09-12 v1
  • Checksum
    3D9E479EB9FF47D2
MKEFYLTVEQIGDSIFERYIDSNGRERTREVEYKPSLFAHCPESQATKYFDIYGKPCTRKLFANMRDASQWIKRMEDIGLEALGMDDFKLAYLSDTYNYEIKYDHTKIRVANFDIEVTSPDGFPEPSQAKHPIDAITHYDSIDDRFYVFDLLNSPYGNVEEWSIEIAAKLQEQGGDEVPSEIIDKIVYMPFDNEKELLMEYLNFWQQKTPVILTGWNVESFDIPYVYNRIKNIFGESTAKRLSPHRKTRVKVIENMYGSREIITLFGISVLDYIDLYKKFSFTNQPSYSLDYISEFELNVGKLKYDGPISKLRESNHQRYISYNIIDVYRVLQIDAKRQFINLSLDMGYYAKIQIQSVFSPIKTWDAIIFNSLKEQNKVIPQGRSHPVQPYPGAFVKEPIPNRYKYVMSFDLTSLYPSIIRQVNISPETIAGTFKVAPLHDYINAIAERPSDVYSCSPNGMMYYKDRDGVVPTEITKVFNQRKEHKGYMLAAQRNCEIIKEALHNPNLSVDEPLDVDYRFDFSDEIKEKIKKLSAKSLNEMLFRAQRTEVAGMTAQINRKLLINSLYGALGNVWFRYYDLRNATAITTFGQMALQWIERKVNEYLNEVCGTEGEAFVLYGDTDSIYVSADKIIDKVGESKFRDTNHWVDFLDKFARERMEPAIDKGFREMCEYMNNKQHLMFMDREAIAGPPLGSKGIGGFWTGKKRYALNVWDMEGTRYAEPKLKIMGLETQKSSTPKAVQKALKECIRRMLQEGEESLQEYFKEFEKEFRQLNYISIASVSSANNIAKYDVGGFPGPKCPFHIRGILTYNRAIKGNIDAPQVVEGEKVYVLPLREGNPFGDKCIAWPSGTEITDLIKDDVLHWMDYTVLLEKTFIKPLEGFTSAAKLDYEKKASLFDMFDF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MH243438
EMBL· GenBank· DDBJ
AWM11695.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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