A0A2U2EA85 · A0A2U2EA85_MYCAV

Function

function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per subunit.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site20-235-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI)
Binding site235-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI)
Binding site1145-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI)
Binding site1195-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI)
Binding site433-435L-homocysteine (UniProtKB | ChEBI)
Binding site433-435L-methionine (UniProtKB | ChEBI)
Binding site486L-homocysteine (UniProtKB | ChEBI)
Binding site486L-methionine (UniProtKB | ChEBI)
Binding site517-5185-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI)
Binding site5635-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI)
Binding site601L-homocysteine (UniProtKB | ChEBI)
Binding site601L-methionine (UniProtKB | ChEBI)
Binding site6075-methyltetrahydropteroyltri-L-glutamate (UniProtKB | ChEBI)
Binding site643Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site643Zn2+ (UniProtKB | ChEBI); catalytic
Binding site645Zn2+ 1 (UniProtKB | ChEBI); catalytic
Binding site645Zn2+ (UniProtKB | ChEBI); catalytic
Binding site658Zn2+ 2 (UniProtKB | ChEBI)
Binding site667Zn2+ (UniProtKB | ChEBI); catalytic
Binding site667Zn2+ 1 (UniProtKB | ChEBI); catalytic
Active site696Proton donor
Binding site728Zn2+ (UniProtKB | ChEBI); catalytic
Binding site728Zn2+ 1 (UniProtKB | ChEBI); catalytic

GO annotations

AspectTerm
Molecular Function5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity
Molecular Functionzinc ion binding
Biological Processmethionine biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
  • EC number
  • Alternative names
    • Cobalamin-independent methionine synthase
    • Methionine synthase, vitamin-B12 independent isozyme

Gene names

    • Name
      metE
    • ORF names
      DFS55_19100

Organism names

  • Taxonomic identifier
  • Strain
    • MAC109
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)

Accessions

  • Primary accession
    A0A2U2EA85

Proteomes

Family & Domains

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    756
  • Mass (Da)
    81,633
  • Last updated
    2018-07-18 v1
  • Checksum
    218F5D3EC8D4ADC3
MTPQAFTATVVGSPRIGPKRELKRATEGYWAGRTGRAELEKVAATLRRDTWTGLAAAGLDSVPVNTFSYYDQMLDTAVMLDALPERARQVSDDLDRYFAAARGNSDVAPLEMTKWFDTNYHYIVPEIAPTTKFALNPDKVLSDLKEALAQGIPARPVVIGPITFLLLSKGVDGAGAPIERLEELVPIYAELLSLLADNGAQWVQLDEPALVTDMCADAPALAEAVYNKLGSAANRPAIYVATYFGDPGASLAGLARTPIEAIGVDLVYGPDTAVAAVPELAGKTVVAGVVDGRNVWRTDLAAALDKLTTLLGSAARVAVSTSCSTLHVPYSLEPETDLDDALRSWLAFGREKVAEVVTLARALRDGREAVADEIAASNAAVASRKSDPRLHNDRVRARIDSIVASGSHRGDPAQRRASQDARLHLPPLPTTTIGSYPQTSAIRKARAALRSGEIDQAEYERRMKKEIADVITLQEQLGLDVLVHGEPERNDMVQYFAEQLEGFFATQNGWVQSYGSRCVRPPILYGDVVRTHPMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQPLADTANQVALAIRDETVDLQAAGIAVIQVDEPALRELLPLRRADQEEYLRWAVGAFRLATSGVADSTQIHTHLCYSEFGEVIGAIADLDADVTSLEAARSHMEVLDDLNAVGFSNSVGPGVYDIHSPRVPSTAEIAESLRAALRAVPAERLWVNPDCGLKTRNPDEVSASLKNMVAAAHEVRAGV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP029332
EMBL· GenBank· DDBJ
AXO24441.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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