A0A2U1Q3L6 · A0A2U1Q3L6_ARTAN
- ProteinATP-dependent 6-phosphofructokinase
- GenePFK
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids893 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 537 | ATP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 600-601 | ATP (UniProtKB | ChEBI) | ||||
Sequence: RG | ||||||
Binding site | 625-628 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GDGT | ||||||
Binding site | 626 | Mg2+ (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Site | 627 | Important for substrate specificity; cannot use PPi as phosphoryl donor | ||||
Sequence: G | ||||||
Binding site | 654-656 | substrate | ||||
Sequence: TID | ||||||
Active site | 656 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 699-701 | substrate | ||||
Sequence: MGR | ||||||
Binding site | 755 | substrate | ||||
Sequence: E | ||||||
Binding site | 812-815 | substrate | ||||
Sequence: YMIR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > campanulids > Asterales > Asteraceae > Asteroideae > Anthemideae > Artemisiinae > Artemisia
Accessions
- Primary accessionA0A2U1Q3L6
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 41-255 | DUF676 | ||||
Sequence: SADHLVVMVNGILGSSEDWRFAAEQFVKSHPDRVFVHRSEKNAASQTLDGVDVMGERLSQEVLEVIKQKPNLRKISFVAHSVGGLVARYAIGRLYRPPREKTEEMSAETFEEEKATIGGLVPMNFITVATPHLGSRGHKQVPFLFGVSALEKVAVLVIRWIFRRTGQHLFLTDDDEGKPPLLKRMLEDSGDCYFMSALRSFHRRVAYANVGYDRI | ||||||
Domain | 530-836 | Phosphofructokinase | ||||
Sequence: ACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIEGGYRGFYSKNTMELTPKIVNDIHKRGGTILQTSRGGHDTNKIVDNIQDRGINQVYIIGGDGTQKGAAAISKEVEKRRLHVAVAGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVDNGVGIVKLMGRYSGFIAMFATLASRDVDCCLIPESPFYLEGEGGLYEYVQQRLKENRHVVIVLAEGAGQEYVSESANANVEKDASGNKLLLDIGLWLTQKIKDHFTTVKKIPINMKYIDPTYMIRAVPSNASDNIYCTLLAQSAVH |
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length893
- Mass (Da)99,523
- Last updated2018-07-18 v1
- Checksum659BC336873537F1
Keywords
- Technical term