A0A2T5IFC4 · A0A2T5IFC4_9PROT

Function

function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site82[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site86[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site89[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site126[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site157[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site217[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site289[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionbiotin synthase activity
Molecular Functioniron ion binding
Biological Processbiotin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Biotin synthase
  • EC number

Gene names

    • Name
      bioB
    • ORF names
      C8R21_10446

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Nl12
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Nitrosomonadales > Nitrosomonadaceae > Nitrosospira

Accessions

  • Primary accession
    A0A2T5IFC4

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-21Disordered
Domain67-294Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    342
  • Mass (Da)
    37,290
  • Last updated
    2018-07-18 v1
  • MD5 Checksum
    393015A1F480094C071689D4DB85010A
MSQSASGVVDMETPSGEAVDSFSDKLSSLGADFRRWDVRRIEALLDLPFSDLIHRAQQVHRQYHDPNGVQLSTLISVKTGGCPEDCGYCPQAARYHTNVENQDMLTVEDVVAAAAAAKAQGATRFCMGAAWRGPRERDMKSVVEMVRAVKALGLEACTTLGMLKPGQAEQLQEAGLDYYNHNLDTSPEFYGEIITTRDYEDRLDTLQKVRHAGINVCCGGIVGMGESRRARAGLIGQLANLDPYPESVPINHLVQVEGTPLHGTEALDPLEFVRTIAAARITMPKAMVRLSAGRREMPEAVQALCFLAGANSIFYGDKLLTTGNPEAERDKALFDKLGLHSI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QAOK01000004
EMBL· GenBank· DDBJ
PTQ82503.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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