A0A2S8JLX6 · A0A2S8JLX6_ECOLX

  • Protein
    Malate synthase G
  • Gene
    glcB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2.

Features

Showing features for binding site, active site.

1723100200300400500600700
TypeIDPosition(s)Description
Binding site118acetyl-CoA (UniProtKB | ChEBI)
Binding site125-126acetyl-CoA (UniProtKB | ChEBI)
Binding site274acetyl-CoA (UniProtKB | ChEBI)
Binding site311acetyl-CoA (UniProtKB | ChEBI)
Active site338Proton acceptor
Binding site338glyoxylate (UniProtKB | ChEBI)
Binding site427Mg2+ (UniProtKB | ChEBI)
Binding site427glyoxylate (UniProtKB | ChEBI)
Binding site452-455glyoxylate (UniProtKB | ChEBI)
Binding site455Mg2+ (UniProtKB | ChEBI)
Binding site536acetyl-CoA (UniProtKB | ChEBI)
Active site631Proton donor

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionmagnesium ion binding
Molecular Functionmalate synthase activity
Biological Processglyoxylate catabolic process
Biological Processglyoxylate cycle
Biological Processtricarboxylic acid cycle

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Malate synthase G
  • EC number

Gene names

    • Name
      glcB
    • ORF names
      NCTC10082_03186

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • NCTC10082
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    A0A2S8JLX6

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue617Cysteine sulfenic acid (-SOH)
Modified residue688Cysteine sulfenic acid (-SOH)

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain16-77Malate synthase N-terminal
Domain158-233Malate synthase G alpha-beta insertion
Domain335-579Malate synthase TIM barrel
Domain591-695Malate synthase C-terminal

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    723
  • Mass (Da)
    80,567
  • Last updated
    2018-07-18 v1
  • Checksum
    CA7D3F0789BDC360
MSQTITQGRLRIDANFKRFVDEEVLPGTGLDTAAFWRNFDEIVHDLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKEAYKSFLRELGYLVPQPERVTVETTGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDPQRGEQVIAWVRRFLDESLPLENGSYQDVLAFKVVDKQLRIQLKNGKETTLRTPAQFVGYRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVAAVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSLLFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPKMHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFINTGFLDRTGDEMHSVMEAGPMLRKNQMKTTPWIKAYERNNVLSGLFCGLRGKAQIGKGMWAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQSEFNAEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIHNVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAGNFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
UFZA01000001
EMBL· GenBank· DDBJ
STE04752.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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