A0A2S5V0D6 · A0A2S5V0D6_9MICO
- ProteinDNA topoisomerase 1
- GenetopA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids965 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
Catalytic activity
Features
Showing features for site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 35 | Interaction with DNA | ||||
Sequence: H | ||||||
Site | 153 | Interaction with DNA | ||||
Sequence: R | ||||||
Site | 154 | Interaction with DNA | ||||
Sequence: R | ||||||
Site | 157 | Interaction with DNA | ||||
Sequence: D | ||||||
Site | 162 | Interaction with DNA | ||||
Sequence: Y | ||||||
Site | 169 | Interaction with DNA | ||||
Sequence: W | ||||||
Active site | 325 | O-(5'-phospho-DNA)-tyrosine intermediate | ||||
Sequence: Y | ||||||
Site | 327 | Interaction with DNA | ||||
Sequence: R | ||||||
Site | 536 | Interaction with DNA | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type I (single strand cut, ATP-independent) activity | |
Molecular Function | metal ion binding | |
Biological Process | DNA topological change |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA topoisomerase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Micrococcales > Microbacteriaceae > Pseudoclavibacter
Accessions
- Primary accessionA0A2S5V0D6
Proteomes
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 5-128 | Toprim | ||||
Sequence: NTLVIVESPTKVKSISQYLGDDYTVLASVGHIRDLIEPKNLPPELKKGSLGKFSVDVDNGFEPYYVVSEQKKKTVAELKRALKDADELILATDEDREGEAIAWHLLQVLQPKVPVKRMVFHEIT | ||||||
Region | 177-182 | Interaction with DNA | ||||
Sequence: SAGRVQ | ||||||
Region | 465-506 | Disordered | ||||
Sequence: KDEARDDSTTAEAKLPPLTKGQKLDPESADAKSHETSPPPRY | ||||||
Compositional bias | 733-754 | Basic and acidic residues | ||||
Sequence: AKAAAAEGRPVKKPRAKKAAAA | ||||||
Region | 733-755 | Disordered | ||||
Sequence: AKAAAAEGRPVKKPRAKKAAAAD | ||||||
Region | 900-965 | Disordered | ||||
Sequence: ADKRAKGPVKRKTTAAKKPAAKKPAAKKPAAAKSAATKTTAAKKPAAKRAPAKKPVAKASEAATTE | ||||||
Compositional bias | 909-923 | Basic residues | ||||
Sequence: KRKTTAAKKPAAKKP |
Sequence similarities
Belongs to the type IA topoisomerase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length965
- Mass (Da)104,596
- Last updated2018-07-18 v1
- Checksum07FA9F394C4D5923
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 733-754 | Basic and acidic residues | ||||
Sequence: AKAAAAEGRPVKKPRAKKAAAA | ||||||
Compositional bias | 909-923 | Basic residues | ||||
Sequence: KRKTTAAKKPAAKKP |