A0A2S5V0D6 · A0A2S5V0D6_9MICO

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

TypeIDPosition(s)Description
Site35Interaction with DNA
Site153Interaction with DNA
Site154Interaction with DNA
Site157Interaction with DNA
Site162Interaction with DNA
Site169Interaction with DNA
Active site325O-(5'-phospho-DNA)-tyrosine intermediate
Site327Interaction with DNA
Site536Interaction with DNA

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      C5B85_10210

Organism names

  • Taxonomic identifier
  • Strain
    • AY1F1
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Micrococcales > Microbacteriaceae > Pseudoclavibacter

Accessions

  • Primary accession
    A0A2S5V0D6

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain5-128Toprim
Region177-182Interaction with DNA
Region465-506Disordered
Compositional bias733-754Basic and acidic residues
Region733-755Disordered
Region900-965Disordered
Compositional bias909-923Basic residues

Sequence similarities

Belongs to the type IA topoisomerase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    965
  • Mass (Da)
    104,596
  • Last updated
    2018-07-18 v1
  • Checksum
    07FA9F394C4D5923
MSGSNTLVIVESPTKVKSISQYLGDDYTVLASVGHIRDLIEPKNLPPELKKGSLGKFSVDVDNGFEPYYVVSEQKKKTVAELKRALKDADELILATDEDREGEAIAWHLLQVLQPKVPVKRMVFHEITREAIEAAKDRTRDIDVHLVDAQETRRILDRLYGYEVSPVLWRKVGPGLSAGRVQSAATRLIVDRERERLAFVKASYWGVEARFAPEQGKEGSAFVARLTKLDGERVATGRDFDDHGVLKNSSVILGEAEATTLTVQLTAGTVPVTVSSVESKPYTRRPAAPFTTSTLQQEAARKLRFTARQTMSVAQSLYENGYITYMRTDSSSLSSQALNAARTQAAKLYGAETVPASPRLYGSKSKNAQEAHEAVRPAGSTFALPSELRPKLSPNDFKLYDLIWKRTIASQMADAKGQTATVTIDASPVDVSDAQGTVTTRVAEFQASGTVITFRGFLAAYEEGKDEARDDSTTAEAKLPPLTKGQKLDPESADAKSHETSPPPRYTEASLVKTLDELGIGRPSTYASIISTIIDRGYVAPRGSALVPSWTAFSVVRLLEEHFHDLVEYNFTAQMEGDLDRIAAGSEQRTDWLRGFYFGTEDHKGLRGVIDNLGEIDAREINSTPLAEGIVLRIGRYGPYLEVAPETEGDTPRRVNVPEDLAPDELTAAKAVELVEAPIQTDRVLGVVPETGKRILVKDGRFGPYVTEGPSEAEEAAAESEAKAKALAEEAEAKAAAAEGRPVKKPRAKKAAAADKPRTASLFKSMIPEQVTYEQAIGLLELPRVVGADSETGAEITAQNGRYGPYLKKGTDTRSLTSEDEIFTVTLEAAHELFAQPKYGARTTKAAIKEFEADPVSGKPIKIKDGRFGPYVTDGETNVTVTRGANPEDVTFEEAVQMLADKRAKGPVKRKTTAAKKPAAKKPAAKKPAAAKSAATKTTAAKKPAAKRAPAKKPVAKASEAATTE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias733-754Basic and acidic residues
Compositional bias909-923Basic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PSTQ01000005
EMBL· GenBank· DDBJ
PPF44506.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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