A0A2S5R6M1 · A0A2S5R6M1_9PROT
- ProteinReplicative DNA helicase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids478 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
The main replicative DNA helicase, it participates in initiation and elongation during chromosome replication. Travels ahead of the DNA replisome, separating dsDNA into templates for DNA synthesis. A processive ATP-dependent 5'-3' DNA helicase it has DNA-dependent ATPase activity.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
- Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | primosome complex | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA binding | |
Molecular Function | DNA helicase activity | |
Biological Process | DNA replication, synthesis of primer | |
Biological Process | DNA unwinding involved in DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameReplicative DNA helicase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Holosporales > Holosporaceae > Holospora
Accessions
- Primary accessionA0A2S5R6M1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Interaction
Subunit
Homohexamer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 183-475 | SF4 helicase | ||||
Sequence: RDSHVVGVSTGLLDLDKMLGGLHPSDLVILAARPAMGKTALATNIAFNAARLKMRDTENKGDGAGVGFFSLEMSALQLAMRIIAQETGVSSDRMRRGAIDKEAFPLFVDLSKELAALPLFIDDTPALSVTSLLTRARRLQRKENIKLIVVDYLQLLTTGKSNSENRVQELSEITRGLKQMAKTLNVPVLALSQLSRAVEQREDKRPMLADLRESGSIEQDADVVMFIYREEYYEARNKPQEGSEKMIHWQRHMAEIYNSAEVIIAKQRHGPTGSVFLQFDGGRTKFSNAASKT |
Sequence similarities
Belongs to the helicase family. DnaB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length478
- Mass (Da)53,520
- Last updated2018-07-18 v1
- Checksum45658D0DF08FC456
Keywords
- Technical term