A0A2S4KHR4 · A0A2S4KHR4_9BURK

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site301Mg2+ (UniProtKB | ChEBI); catalytic
Binding site344Mg2+ (UniProtKB | ChEBI); catalytic
Binding site402Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site405Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      BV908_05145

Organism names

  • Taxonomic identifier
  • Strain
    • LR2014-1
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Comamonadaceae > Diaphorobacter

Accessions

  • Primary accession
    A0A2S4KHR4

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-118S1 motif
Region402-405Required for zinc-mediated homotetramerization and catalytic activity
Region507-576Disordered
Region600-937Disordered
Compositional bias614-707Basic and acidic residues
Compositional bias715-729Polar residues
Compositional bias733-774Basic and acidic residues
Compositional bias813-840Basic and acidic residues
Compositional bias909-927Pro residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,029
  • Mass (Da)
    111,485
  • Last updated
    2018-07-18 v1
  • Checksum
    7613AA66AEE6B24E
MKRMLINATQPEERRLAIVDGQKLLDYEIEIEGREQRKGNIYKAVVTRVEPSLEACFVDYGEDRHGFLPFKEISKQYFAEGVSPSQARIQDVIKEGQELIVQVEKEERGNKGAALTTFISLAGRYVVLMPNNPRGGGVSRRIEGEDRAELKEAMDQLEYPKGMSIIARTAGIGRSAPELQWDLNYLLKLWTAIDGAAKAGKGAFLIYQESSLVIRAIRDYFNSDIGDILIDTDDIYEQAHQFMAHVMPEHAGKVKRYRDDAPLFSRFQIEHQIESAYARTVTLPSGGAIVIDHTEALVSVDVNSARAIKGGDIEETATRTNLEAADEVARQMRLRDLGGLIVIDFIDMEESKNRREVENRLRDALRQDRARVQFGTISKFGLMEMSRQRLKPSLSEGAHIRCPLCDGTGHVRDTEGFSLQMLRMIQEESMKDSTAAVHCQVPVEVASFLLNEKRTEIAKIELKQRVSVVVIPNKSLETPHYKLERLKHDDPRLENLDASYKLADVEEDQTKVTRRSQEPTNKQTPVIKGVLPDAPAPVAEPRPEGAARGATRPQGQGQQRPATAPTPAPQRPAAAPAPEQGFFAWIKSLFGFGAAPAPAPVAAPAQTPAEPGARGEGRRGERGGRRGERTGERSGERGGERNGNREGGEGRGRRGEGRGGERSAEGRENREPREPRNGRDGERRGDRAEGESRREPREGRGGRERGERNAQPPVQSAADLANSPVTTDADVAVTEERTARTGERGERRERGERGERRERGGERGGERRERGPRTIDANAPAAGADFADTAPLAALPDLDLSGAEVGTSGAEAPAGEERRERRSRDRYGRDRRERGERGPRNAEASATGADAEAPGAPLMDLDLPAPAAPAEEDSAPARRSYFASAPASAPAATPQAASEAAAAVTPPPAAVAAPAPAPAPVAAAPVPAPAPAATAVAPATGGMPRVQAFTLPVDTLQQVAQASGLQWVNSDAQKIAAAQAAIAAEPKPVHVPRERAPVVVLDEGPLVLVETRRDLGAMQLPFDQDNAAA

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias614-707Basic and acidic residues
Compositional bias715-729Polar residues
Compositional bias733-774Basic and acidic residues
Compositional bias813-840Basic and acidic residues
Compositional bias909-927Pro residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MTBE01000004
EMBL· GenBank· DDBJ
POR11682.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp