A0A2R6RB93 · A0A2R6RB93_ACTCC

Function

function

Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Activity regulation

Allosterically activated by fructose 2,6-bisphosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site105diphosphate (UniProtKB | ChEBI)
Binding site199Mg2+ (UniProtKB | ChEBI); catalytic
Site200Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP
Site226Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi
Binding site227-229substrate
Active site229Proton acceptor
Binding site266-267substrate; ligand shared between dimeric partners
Binding site274-276substrate
Binding site335substrate
Binding site440-443substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function6-phosphofructokinase activity
Molecular FunctionATP binding
Molecular Functiondiphosphate-fructose-6-phosphate 1-phosphotransferase activity
Molecular Functionmetal ion binding
Biological Processfructose 6-phosphate metabolic process
Biological Processphotosynthesis
Biological Processresponse to glucose

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
  • EC number
  • Short names
    PFP
  • Alternative names
    • 6-phosphofructokinase, pyrophosphate dependent
    • PPi-PFK
    • Pyrophosphate-dependent 6-phosphofructose-1-kinase

Gene names

    • Name
      PFP-BETA
    • ORF names
      CEY00_Acc09725

Organism names

Accessions

  • Primary accession
    A0A2R6RB93

Proteomes

Genome annotation databases

Subcellular Location

Keywords

Interaction

Subunit

Tetramer of two alpha (regulatory) and two beta (catalytic) chains.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain97-462Phosphofructokinase

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    567
  • Mass (Da)
    61,742
  • Last updated
    2018-06-20 v1
  • Checksum
    836EB6A67A2FEBD4
MATALVANGDFSSGKSPDPVTGRFAAVYSEVQNSRLDHSLPLPSVLQKPFKVVAGPPSSAAGNPDEIAKLFPCLFGQPSAMLVPGDSAELSSSQSLKIGVVLSGGQAPGGHNVISGVFDYLQSRCKGSTLYGFRGGPAGIMKCKYVVLTPEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKARVIGCPKTIDGDLKSKEVPISFGFDTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITLIGEEVAAKQQTLKNVTDYIVDAICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHEVVDEGGAWKKKLKVESLQLFELLPPAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEHRKQMGAYKDQFKGQSHFFGYEGRCGMPSNFDSTYCYALGFAAGALLHSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASMREEWALNNRYISPGPIQFIGPVADKVNHTLLLELGIHA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NKQK01000008
EMBL· GenBank· DDBJ
PSS24809.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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