A0A2R6D1S9 · A0A2R6D1S9_9EURY

Function

function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ ion per subunit.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site136Fe cation (UniProtKB | ChEBI)
Binding site140Fe cation (UniProtKB | ChEBI)
Binding site157-161L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site189L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site202L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site206L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site301L-threonylcarbamoyladenylate (UniProtKB | ChEBI)
Binding site329Fe cation (UniProtKB | ChEBI)
Binding site393-401ATP (UniProtKB | ChEBI)
Binding site410ATP (UniProtKB | ChEBI)
Active site499Proton acceptor; for kinase activity

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular ComponentEKC/KEOPS complex
Molecular FunctionATP binding
Molecular Functionhistone H2AS1 kinase activity
Molecular Functioniron ion binding
Molecular Functionmetalloendopeptidase activity
Molecular FunctionN(6)-L-threonylcarbamoyladenine synthase activity
Molecular Functionprotein serine kinase activity
Molecular Functionprotein serine/threonine/tyrosine kinase activity
Molecular Functionzinc ion binding
Biological Processphosphorylation
Biological ProcesstRNA threonylcarbamoyladenosine modification

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including 2 domains:

  • Recommended name
    tRNA N6-adenosine threonylcarbamoyltransferase
  • EC number
  • Alternative names
    • tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
    • t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
  • Recommended name
    Serine/threonine-protein kinase Bud32
  • EC number

Gene names

    • ORF names
      BRC66_07845

Organism names

Accessions

  • Primary accession
    A0A2R6D1S9

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.

Family & Domains

Features

Showing features for region, compositional bias, domain.

Type
IDPosition(s)Description
Region1-368Kae1
Compositional bias71-85Basic and acidic residues
Region71-96Disordered
Domain92-335Gcp-like
Region242-261Disordered

Sequence similarities

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.
In the N-terminal section; belongs to the KAE1 / TsaD family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    587
  • Mass (Da)
    62,716
  • Last updated
    2018-06-20 v1
  • Checksum
    91F3DD4B8D626905
MAGDSLGDVRVLGIEGTAWAASAALYDAASDTVDIETAAYQPESGGIHPREAAEHMADHLPAVIERMLERARETADGDREPPVDVAERSSSGQQAAPVDAVAFSRGPGLGPCLRLAGTSARALAQRLDVPLIGVNHMVAHLEIGRHRSGFSSPVCLNASGANAHILGYRNGRYRVLGETMDTGVGNAIDKFTRHVGWSHPGGPKVEAAAEDGEYVDLPYVVKGMDFSFSGIMSAAKQAYDGGPASDASGASSDQRSGGEPVENVCCGLQENVFAMLAEVSERALSLTGSDELVLGGGVGQNDRLQSMLASMCEQRGADFFAPEDRFLRDNAGMIAVLGAKMHAAGDTLPVEQSGIDSEFRPDEVPVTWRGADEAPVTWRGGEKAVGRPVDGDTVQGAEATVTIEPDRVVKRRLPREYRHPTLDARLRRGRTRQEARLTSEARGVGVPTPVIRDVDLGETTLVFQRVGDCDLREALTAGRVRAVARHLARLHDAGVVHGDPTTRNVRVDRTDGTGDGDRVFLIDFGLGYYTTDPEDHAMDLHVLAQSLAGTDDDADRLQRAAEDAYRTVGDGAVLDQLREIEGRGRYQ

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias71-85Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PXQP01000204
EMBL· GenBank· DDBJ
PSP36877.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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