A0A2P1JNM1 · A0A2P1JNM1_LASV

  • Protein
    Pre-glycoprotein polyprotein GP complex
  • Gene
    GPC
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    5/5

Function

function

Glycoprotein G1

Forms the virion spikes together with glycoprotein G2. The glycoprotein spike trimers are connected to the underlying matrix. Interacts with the host receptor. Mediates virus attachment to the host primary receptor alpha-dystroglycan DAG1 (alpha-DG) at the cell surface. This attachment induces virion internalization apparently through macropinocytosis. Following endocytosis, there is a pH-dependent switch from binding DAG1 to the host lysosomal receptor LAMP1. This latter binding triggers the dissociation of GP1, exposing the fusion subunit, GP2, such that fusion can occur. Down-modulates host DAG1.

Glycoprotein G2

Forms the virion spikes together with glycoprotein G1. The glycoprotein spike trimers are connected to the underlying matrix. Class I viral fusion protein that directs fusion of viral and host endosomal membranes, leading to delivery of the nucleocapsid into the cytoplasm. Membrane fusion is mediated by irreversible conformational changes induced by acidification.

Stable signal peptide

Functions as a cleaved signal peptide that is retained as the third component of the GP complex (GP-C). Helps to stabilize the spike complex in its native conformation. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of G1 and G2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Features

Showing features for binding site, site.

TypeIDPosition(s)Description
Binding site57Zn2+ 1 (UniProtKB | ChEBI)
Site58-59Cleavage; by host signal peptidase
Site91Binding to the host receptor LAMP1
Site92Binding to the host receptor LAMP1
Site229Binding to the host receptor LAMP1
Site258-259Cleavage; by host MBTPS1
Binding site454Zn2+ 2 (UniProtKB | ChEBI)
Binding site456Zn2+ 2 (UniProtKB | ChEBI)
Binding site462Zn2+ 2 (UniProtKB | ChEBI)
Binding site466Zn2+ 1 (UniProtKB | ChEBI)
Binding site474Zn2+ 1 (UniProtKB | ChEBI)
Binding site476Zn2+ 1 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componenthost cell endoplasmic reticulum membrane
Cellular Componenthost cell Golgi membrane
Cellular Componenthost cell plasma membrane
Cellular Componentmembrane
Cellular Componentviral envelope
Cellular Componentvirion membrane
Molecular Functionmetal ion binding
Biological Processfusion of virus membrane with host endosome membrane
Biological Processreceptor-mediated endocytosis of virus by host cell
Biological Processvirion attachment to host cell

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Pre-glycoprotein polyprotein GP complex
  • Short names
    Pre-GP-C
  • Cleaved into 3 chains

Gene names

    • Name
      GPC

Organism names

Accessions

  • Primary accession
    A0A2P1JNM1

Subcellular Location

Glycoprotein G2

Virion membrane
; Single-pass membrane protein
Host endoplasmic reticulum membrane
; Single-pass membrane protein
Host Golgi apparatus membrane
; Single-pass membrane protein
Host cell membrane
; Single-pass membrane protein
Note: Binding to the stable signal peptide masks endogenous ER localization signals in the cytoplasmic domain of G2 to ensure that only the fully assembled, tripartite GP complex is transported for virion assembly.

Glycoprotein G1

Virion membrane
; Peripheral membrane protein
Host endoplasmic reticulum membrane
; Peripheral membrane protein
Host Golgi apparatus membrane
; Peripheral membrane protein
Host cell membrane
; Peripheral membrane protein

Stable signal peptide

Virion membrane
; Single-pass type II membrane protein
Host endoplasmic reticulum membrane
; Single-pass type II membrane protein
Host Golgi apparatus membrane
; Single-pass type II membrane protein
Host cell membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain2-17Extracellular
Transmembrane21-52Helical
Topological domain34-58Cytoplasmic
Transmembrane427-449Helical
Topological domain453-490Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for initiator methionine, lipidation, chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
Initiator methionine1Removed; by host
Lipidation2N-myristoyl glycine; by host
ChainPRO_50233164342-58Stable signal peptide
ChainPRO_50233164352-490Pre-glycoprotein polyprotein GP complex
Glycosylation78N-linked (GlcNAc...) asparagine; by host
Disulfide bond85↔230
Glycosylation88N-linked (GlcNAc...) asparagine; by host
Glycosylation98N-linked (GlcNAc...) asparagine; by host
Glycosylation108N-linked (GlcNAc...) asparagine; by host
Disulfide bond117↔154
Glycosylation118N-linked (GlcNAc...) asparagine; by host
Glycosylation166N-linked (GlcNAc...) asparagine; by host
Disulfide bond179↔211
Glycosylation223N-linked (GlcNAc...) asparagine; by host
ChainPRO_5023316436259-490Glycoprotein G2
Disulfide bond278↔291
Disulfide bond300↔309
Disulfide bond363↔384
Glycosylation364N-linked (GlcNAc...) asparagine; by host
Glycosylation372N-linked (GlcNAc...) asparagine; by host
Glycosylation389N-linked (GlcNAc...) asparagine; by host
Glycosylation394N-linked (GlcNAc...) asparagine; by host

Post-translational modification

Pre-glycoprotein polyprotein GP complex

Specific enzymatic cleavages in vivo yield mature proteins. GP-C polyprotein is cleaved in the endoplasmic reticulum by the host protease MBTPS1. Only cleaved glycoprotein is incorporated into virions. The SSP remains stably associated with the GP complex following cleavage by signal peptidase.

Keywords

Interaction

Subunit

Glycoprotein G1

Homotrimer; disulfide-linked. In pre-fusion state, G1 homotrimers bind G2 homotrimers via ionic interactions. Part of the GP complex (GP-C) together with glycoprotein G2 and the stable signal peptide. Interacts with the primary host receptor DAG1 on the cell surface; this interaction occurs at pH 8.0 but not at pH 6.0 and below. Upon virus internalization and at endosomal pH, interacts with the host lysosomal protein LAMP1; this interaction mediates G1 dissociation from GP-C and membrane fusion. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding.

Glycoprotein G2

Homotrimer. Interacts with the stable signal peptide. In pre-fusion state, G2 homotrimers bind G1 homotrimers via ionic interactions. Part of the GP complex (GP-C) together with glycoprotein G1 and the stable signal peptide. Acidification in the endosome triggers rearrangements, which ultimately leads to a 6 helix bundle formed by the two heptad repeat domains (HR1 and HR2) in post-fusion state. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding.

Stable signal peptide

Interacts with glycoprotein G2. Part of the GP complex (GP-C) together with glycoprotein G1 and glycoprotein G2. The GP-complex interacts with protein Z, which interacts with ribonucleocapsid; these interactions may induce virion budding.

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region187-215Binding to the host receptor LAMP1
Region257-293Fusion
Region294-362HR1
Region367-430HR2

Domain

Glycoprotein G1

Upon protonation in a weak acidic environment, the histidine triad involved in host LAMP1 binding inhibits pre-mature triggering of the spike, an inhibition that LAMP1 overrides.

Glycoprotein G2

Contains 1 fusion peptide at the N-terminus, 2 heptad repeats domains HR1 and HR2 and, at the C-terminus, a cytoplasmic domain that plays a role in ER location. Also contains a zinc-binding domain that allows SSP retention in the GPC complex by accepting a cysteine from SSP as the fourth ligand.

Stable signal peptide

The N-terminus is localized at the extracellular side of the GP-C, with a part embedded in the membrane probably.

Sequence similarities

Belongs to the arenaviridae GPC protein family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    490
  • Mass (Da)
    55,712
  • Last updated
    2018-05-23 v1
  • Checksum
    D6EE98E4F3FF3895
MGQIITFFQEVPHVIEEVMNIVLIALSLLAILKGIYNVATCGLFGLVSFLLLCGRSCSTTYKGVYELQTLELDMANLNMTMPLSCTKNNSHHYIMVGNETGLELTLTNTSIINHKFCNLSDAHKKNLYDHALMSIISTFHLSIPNFNQYEAMSCDFNGGKISVQYNLSHTYAVDAANHCGTIANGVLQTFMRMAWGGSYIALDSGKGSWDCIMTSYQYLVIQNTTWEDHCQFSRPSPIGYLGLLSQRTRDIYISRRLLGTFTWTLSDSEGNETPGGYCLTRWMLIEAELKCFGNTAVAKCNEKHDEEFCDMLRLFDFNKQAIRRLKTEAQMSIQLINKAVNALINDQLIMKNHLRDIMGIPYCNYSKYWYLNHTVTGKTSLPRCWLVSNGSYLNETHFSDDIEQQADNMITELLQKEYIDRQGKTPLGLVDLFVFSTSFYLISIFLHLVKIPTHRHITGKPCPKPHRLNHMGICSCGLYKHPGVPVKWKR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MH053573
EMBL· GenBank· DDBJ
AVO03639.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

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