A0A2P1DP98 · MACF_PENTR

  • Protein
    FAD-dependent monooxygenase macF
  • Gene
    macF
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of macrophorins, isoprenoid epoxycyclohexenones containing cyclized drimane moieties (PubMed:28926261).
The first step of the pathway is the synthesis of 6-methylsalicylic acid (6-MSA) by the polyketide synthase macA (PubMed:28926261).
6-MSA is then converted to m-cresol by the decarboxylase macB (By similarity).
The cytochrome P450 monooxygenase macC then catalyzes the oxidation of m-cresol to toluquinol (By similarity).
Epoxidation of toluquinol is then performed by the short chain dehydrogenase macD, with the help of macE, and a further prenylation by macG leads to 7-deacetoxyyanuthone A (By similarity).
The next step is the hydroxylation of C-22 of 7-deacetoxyyanuthone A by the cytochrome P450 monooxygenase macH to yield 22-deacetylyanuthone A (By similarity).
O-Mevalon transferase macI then attaches mevalon to the hydroxyl group of 22-deacetylyanuthone A to produce yanuthone E (By similarity).
The terpene cyclase macJ catalyzes the cyclization of 22-deacetylyanuthone A to macrophorin A (PubMed:28926261).
MacJ is also able to catalyze cyclization of yanuthone E and 7-deacetoxyyanuthone A to their corresponding macrophorins (PubMed:28926261).
The macJ products can be further modified by macH and macJ, as well as by the FAD-dependent monooxygenase macF, to produce additional macrophorins, including 4'-oxomacrophorin A, 4'-oxomacrophorin D and 4'-oxomacrophorin E (PubMed:28926261).

Miscellaneous

The macrophorins cluster contains a single gene insertion (encoding for the terpene cyclase macJ) compared with the yanuthone cluster that produces the linear compound yanuthone.

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

GO annotations

AspectTerm
Molecular FunctionFAD binding
Molecular Functionoxidoreductase activity
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    FAD-dependent monooxygenase macF
  • EC number
  • Alternative names
    • Macrophorins biosynthesis cluster protein F

Gene names

    • Name
      macF

Organism names

  • Taxonomic identifier
  • Strain
    • LM2
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium

Accessions

  • Primary accession
    A0A2P1DP98

PTM/Processing

Features

Showing features for signal, chain, modified residue.

TypeIDPosition(s)Description
Signal1-20
ChainPRO_501513641121-519FAD-dependent monooxygenase macF
Modified residue125Pros-8alpha-FAD histidine

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain88-262FAD-binding PCMH-type

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    519
  • Mass (Da)
    55,144
  • Last updated
    2018-05-23 v1
  • Checksum
    3975E83101269FC5
MTKMTSIIGILMGVLTTATAASIPTGSSAAAAAALGSLGVSPPSGNVLIGNAGYTCSLLNRVLSKNETFTVTSPYYDVLIDEAWSENCRLNASCIVTPESAEEVSRLLQILSILETRFAIRSGGHNTNPGFSSIGSDGVLIALEKLDSISLSADRGTVTVGPGNKWESVYKYLQPYNLTALGGREAVVGVGGYILGETGGLSTFYNTHGLAIDSVTRFQVVLPNGTIVDATPTEHADLYKGLKGGLNNFGIVTEYDLTTNTGVDIYYEIKTYTTANTPAVLAAYATYLLDADINSNVEIQINPSYTLVFYGYLGHVSAPTDFDPFSDIPVASTMYPPTNGSLTELLLSIGSTGLTSEGVSYSGTFSFKVTGSTFLQDTYSTYLEAAASLPTGAVLSYVPQGVIPNLVTQGKSQNGGNLLGLDATPQVWANIFVQFPATLSQSEVAGSVDSLLANLISSAKSEDLFLPYIFVNDAGAKQKPLQSFGEKNIKYIDTVAKRYDPKRIMQRLQNQAYFVLEEL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MF990005
EMBL· GenBank· DDBJ
AVK70106.1
EMBL· GenBank· DDBJ
Genomic DNA
MH388470
EMBL· GenBank· DDBJ
QBC75442.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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