A0A2P1DP94 · MACC_PENTR

  • Protein
    Cytochrome P450 monooxygenase macC
  • Gene
    macC
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of macrophorins, isoprenoid epoxycyclohexenones containing cyclized drimane moieties (PubMed:28926261).
The first step of the pathway is the synthesis of 6-methylsalicylic acid (6-MSA) by the polyketide synthase macA (PubMed:28926261).
6-MSA is then converted to m-cresol by the decarboxylase macB (By similarity).
The cytochrome P450 monooxygenase macC then catalyzes the oxidation of m-cresol to toluquinol (By similarity).
Epoxidation of toluquinol is then performed by the short chain dehydrogenase macD, with the help of macE, and a further prenylation by macG leads to 7-deacetoxyyanuthone A (By similarity).
The next step is the hydroxylation of C-22 of 7-deacetoxyyanuthone A by the cytochrome P450 monooxygenase macH to yield 22-deacetylyanuthone A (By similarity).
O-Mevalon transferase macI then attaches mevalon to the hydroxyl group of 22-deacetylyanuthone A to produce yanuthone E (By similarity).
The terpene cyclase macJ catalyzes the cyclization of 22-deacetylyanuthone A to macrophorin A (PubMed:28926261).
MacJ is also able to catalyze cyclization of yanuthone E and 7-deacetoxyyanuthone A to their corresponding macrophorins (PubMed:28926261).
The macJ products can be further modified by macH and macJ, as well as by the FAD-dependent monooxygenase macF, to produce additional macrophorins, including 4'-oxomacrophorin A, 4'-oxomacrophorin D and 4'-oxomacrophorin E (PubMed:28926261).

Miscellaneous

The macrophorins cluster contains a single gene insertion (encoding for the terpene cyclase macJ) compared with the yanuthone cluster that produces the linear compound yanuthone.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Secondary metabolite biosynthesis; terpenoid biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site448Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Biological Processmycotoxin biosynthetic process
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 monooxygenase macC
  • EC number
  • Alternative names
    • Macrophorins biosynthesis cluster protein C

Gene names

    • Name
      macC

Organism names

  • Taxonomic identifier
  • Strain
    • LM2
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium

Accessions

  • Primary accession
    A0A2P1DP94

Subcellular Location

Membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane2-22Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004540861-536Cytochrome P450 monooxygenase macC

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    536
  • Mass (Da)
    61,412
  • Last updated
    2018-05-23 v1
  • Checksum
    C980E6537D1E4DA4
MALLYITTAALALLLLFLRAVFKSWRIQRKLPPGPPGLPLIGNIHQIPAVRAHQKFTEWAKVYGGLYTFRIGPATAAVITDRGLVKELLDKRSALYSSRPVSYVGQNLITGGDHLLLMDNNEMWRLFRKTVHQHFKASMCEKEHVKLLEAEHTQMMRDFLLYPEKHMLHTKRTTNSIIMSLLYGIRTPSWDTPHMRELYEIMEQWSKVMETGATPPVDIFPWLRWIPQRWLGNWVDRSVEVGSGMKALYGSFRRRAIEARREAEQSSQSRARTFIDHVLDLQEKANLTDNQVDFLGGVMMEGGSDTGSTMLLVMIQALVRYPEVQERARAELDAVCGEGRSPTWADFSRLPYINMIVKETMRWRPVTPLSFPHALNQDDWVNGYLLPKGTTVFLNVWGLHHDESIFPNPERFDPSHYEGRHNLASDYAASPDYMQRDHFIYGAGRRLCPGIHLSERSMFIGAAKLLWCFQFEPEMDESGRPVAIDTDPITGYTEGFLVCPQAYKCKVSPRSTARAETIMREFAQAESEVLCQYATP

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MF989996
EMBL· GenBank· DDBJ
AVK70097.1
EMBL· GenBank· DDBJ
Genomic DNA
MH388470
EMBL· GenBank· DDBJ
QBC75451.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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