A0A2N7YCR5 · A0A2N7YCR5_9PSED

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site297Mg2+ (UniProtKB | ChEBI); catalytic
Binding site340Mg2+ (UniProtKB | ChEBI); catalytic
Binding site398Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site401Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      C1X83_02850

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • GP01-A4
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas

Accessions

  • Primary accession
    A0A2N7YCR5

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain36-114S1 motif
Region398-401Required for zinc-mediated homotetramerization and catalytic activity
Compositional bias585-679Basic and acidic residues
Region585-796Disordered
Compositional bias699-727Basic and acidic residues
Region1038-1084Disordered
Compositional bias1057-1084Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,084
  • Mass (Da)
    118,747
  • Last updated
    2018-04-25 v1
  • Checksum
    DE5A652A8DB40D84
MLINATQPEELRVALVDGQRLYDLDIESGAREQKKANIYKGRITRIEPSLEAAFVDFGSERHGFLPLKEISREYFKKSPEGRVNIKDVLSEGQEVIVQVEKEERGNKGAALTTFISLAGRYLVLMPNNPRAGGISRRIEGEERNELREALNGLIAPADMGLIVRTAGLGRSSEEMQWDLDYLLQLWTAIKEASLDRSAPFLIYQESNVIIRAIRDYLRQDIGEVLIDSVEAQDEALTFIRQVMPQYASKIKLYEDSVPLFNRFQIESQIETAFQRVVELPSGGSIVIDPTEALVSIDINSARATKGSDIEETALQTNLEAAEEIARQLRLRDIGGLIVIDFIDMTPAKNQRAVEEKVRECLEADRARVQVGRISRFGLLEMSRQRLRPSLGESSGIVCPRCNGTGIIRDVESLSLAILRLIEEEALKDRTAEVRAQVPIPVAAFLLNEKRNSITKIELRTRARIVILPNDHLETPHFEVQRLRDDSPEAHVNQSSYEIAAAAAEVEEVQPAAATRTLVRQEAAVKTAPARANAPVPVEAAAPVAAPAALPEPSLFKGLVKSLVSLFATKEEPVAPVVVEKPVTERPARNEERRNGRQQSRNRNGRRDEERKPREERAPREERAPREERQPREAREETPTVAREERAPREERAPRAPRAPREDRKPRGEREERVRELREPLDAAPAVAAAAATAATAEERPARQPREERAPRPPREERQPRAEQAAPAGEEEELLSNEDQQQEDGQDNAEGDRPRRRSRGQRRRSNRRERQRDANGNEIEGSEETGEANAAATGEPTGAELAAGLAVTAAVASSVISAPAEAQANEQAEAATAAIQENTVVETPAVEAPVVEATTPVEAPVVPEVEVAPARHVREVREVREPQPEIDVVAEPAVVVEQPPVVEAAVEAPVAEPTPEVREVREEQTAFQWSAEPAPVVEAVEAPAPAPVVEEAPVPVAEVVVAAEPVAVVAEPAPVVEAPVVAEVAAPVVEAAPASALTENGRAPNDPREVRRRRKEAEAAAAAAKAAAEAAPAVAEVAEAAPAPVTEAVVEHSAPAIDENVRSVEEVVEHHPKAQEEEHEPKPLV

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias585-679Basic and acidic residues
Compositional bias699-727Basic and acidic residues
Compositional bias1057-1084Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PODW01000002
EMBL· GenBank· DDBJ
PMV15252.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp