A0A2N7BG60 · A0A2N7BG60_9BACT

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

TypeIDPosition(s)Description
Site34Interaction with DNA
Site139Interaction with DNA
Site140Interaction with DNA
Site143Interaction with DNA
Site155Interaction with DNA
Active site284O-(5'-phospho-DNA)-tyrosine intermediate
Site286Interaction with DNA
Site473Interaction with DNA

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      BWI97_03250

Organism names

  • Taxonomic identifier
  • Strain
    • BAB-5405
  • Taxonomic lineage
    Bacteria > Bacteroidota > Cytophagia > Cytophagales > Cytophagaceae > Siphonobacter

Accessions

  • Primary accession
    A0A2N7BG60

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain4-114Toprim
Region163-168Interaction with DNA
Region323-343Disordered
Region815-835Disordered

Sequence similarities

Belongs to the type IA topoisomerase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    835
  • Mass (Da)
    93,813
  • Last updated
    2018-04-25 v1
  • Checksum
    31AED131FA14F372
MAKKNLVIVESPAKAKTIEGYLGADFQVKSSYGHVRDLPDKGLAIDIKNGFLPTYEVSPEKIKVVNELRQLAKSSQEVWLATDDDREGEAISWHLKETLGLPDTTKRIVFREITKTAIQKAIQSPRTINQDLVNAQQARRVLDRLIGFELSPVLWEKVKRGLSAGRVQSVAVRLVVERERSIDQHQSKSSFKVVAQFLLDGNKVLQAELPKNFETEAQARAFLEKCKGATFSIRNLETKPAKKSPAPPFTTSTLQQEASRKLGFAVAATMQYAQKLYEAGKISYMRTDSTNLSEEALAKSKASIIEQFGEKYSQQRRYKTKNDSAQEAHEAIRPTDFSVPSAGETRQEQRLYELIWKRGIASQMADAQLERTTATIGISTTSEELIAQGEVIKFDGFLKVYLESKDDEDEESKGMLPPLSIGQILNLDVLKATERFSRPPARFTEASLVKELEERGIGRPSTYAPTISTIIKREYVVKEDRPGKERNFKELTLKNDELTEKTGKETYGTEKAKLFPTTIGMIVNDFLVDNFDNVVDFKFTADMEEEFDKIAEGQIEWQRMIDNFYKNFHLTIEQSSSAERSSVKSARELGIDPKTGRMVSVRLGKYGPFAQLGDSKEEQKPQYAPLPKDKLLESITLEEAIELFKLPRTVGELEGKELLVNIGKLGPYIKFDEKYYNLSEGDDPYTIDEERAIQVIQEKRAAEASDAIGEFEGQPISKGKGRFGPYVKHDGKFYSLPRGMNLDTITLAEAIQIIQNKRTAEANKIIKEFPENTTVKVLNGQYGPYIQIGKRNVKIPKGTEPASLTLEQCLELGGESPAAATEEKKTTRKKAAPKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NJAG01000003
EMBL· GenBank· DDBJ
PMD98844.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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