A0A2N2QZI7 · A0A2N2QZI7_9PROT

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site70-83NAD+ (UniProtKB | ChEBI)
Binding site90substrate
Binding site100substrate
Binding site128substrate
Site135Important for catalysis
Site185Important for catalysis
Binding site217Mg2+ (UniProtKB | ChEBI)
Binding site217substrate
Binding site241Mg2+ (UniProtKB | ChEBI)
Binding site245Mg2+ (UniProtKB | ChEBI)
Binding site275-287NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • ORF names
      CVU33_20135

Organism names

Accessions

  • Primary accession
    A0A2N2QZI7

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain2-346Isopropylmalate dehydrogenase-like

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    350
  • Mass (Da)
    37,803
  • Last updated
    2018-04-25 v1
  • Checksum
    99F0AE5936BFA473
MKICVLPGDGIGPEIVAEAVRVLKSLDLKLELEEGLIGGGAVDATGTPYPEATKKLTAEADAILLGAVGGPKWDVLARELRPERGLLGIRKDLGLFANLRPAILYPELANASTLKPEVVSGLDILIVRELTGDIYFGQPRGIREENGERVGFNTMVYSESEIRRIGHVAFQAAQKRNKKLCSVDKMNVLETTQLWRDVMIEIAHDYPDVELSHMLVDNAAMQLVKAPKQFDVMVTGNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPSHGSAPDIAGQGVANPLATILSVAMLLRYTFGLEEQALRVENAVKRVLAQGYRTGDIYERGTNKVGTKEMGDAVLAAL

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PHCF01000184
EMBL· GenBank· DDBJ
PKO36114.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help