A0A2N2QZI7 · A0A2N2QZI7_9PROT
- Protein3-isopropylmalate dehydrogenase
- GeneleuB
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids350 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity
- (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
Cofactor
Protein has several cofactor binding sites:
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 70-83 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 90 | substrate | |||
Binding site | 100 | substrate | |||
Binding site | 128 | substrate | |||
Site | 135 | Important for catalysis | |||
Site | 185 | Important for catalysis | |||
Binding site | 217 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 217 | substrate | |||
Binding site | 241 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 245 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 275-287 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | 3-isopropylmalate dehydrogenase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | NAD binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-isopropylmalate dehydrogenase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria
Accessions
- Primary accessionA0A2N2QZI7
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-346 | Isopropylmalate dehydrogenase-like | |||
Sequence similarities
Family and domain databases
Sequence
- Sequence statusComplete
- Length350
- Mass (Da)37,803
- Last updated2018-04-25 v1
- Checksum99F0AE5936BFA473
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
PHCF01000184 EMBL· GenBank· DDBJ | PKO36114.1 EMBL· GenBank· DDBJ | Genomic DNA |