A0A2N0UL18 · A0A2N0UL18_9FIRM
- ProteinMethylated-DNA--protein-cysteine methyltransferase
- GeneadaB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids174 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Miscellaneous
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
Catalytic activity
- a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 134 | Nucleophile; methyl group acceptor | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | methylated-DNA-[protein]-cysteine S-methyltransferase activity | |
Biological Process | DNA alkylation repair | |
Biological Process | methylation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMethylated-DNA--protein-cysteine methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Clostridia > Eubacteriales > Oscillospiraceae > Ruminococcus
Accessions
- Primary accessionA0A2N0UL18
Proteomes
Subcellular Location
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 4-73 | Methylguanine DNA methyltransferase ribonuclease-like | ||||
Sequence: TDYYNSPIGGITMASEGEGLCGLWFDGQKYFASTVKGETEDKSLPVFEKTKKWLDIYFSGEEPDFTPKLS | ||||||
Domain | 78-162 | Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding | ||||
Sequence: KFRKAVWDILLTIPYGSVMTYGEIARIMAKQSGIAKMSAQAVGGAVGHNPVSIIVPCHRVVGTNGNLTGYAGGIDKKISLLKNEK |
Sequence similarities
Belongs to the MGMT family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length174
- Mass (Da)19,014
- Last updated2018-04-25 v1
- Checksum3632194581519EC4
Keywords
- Technical term