A0A2N0UL18 · A0A2N0UL18_9FIRM

  • Protein
    Methylated-DNA--protein-cysteine methyltransferase
  • Gene
    adaB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site134Nucleophile; methyl group acceptor

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionmethylated-DNA-[protein]-cysteine S-methyltransferase activity
Biological ProcessDNA alkylation repair
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Methylated-DNA--protein-cysteine methyltransferase
  • EC number
  • Alternative names
    • 6-O-methylguanine-DNA methyltransferase
      (MGMT
      )
    • O-6-methylguanine-DNA-alkyltransferase

Gene names

    • Name
      adaB
    • ORF names
      RBATCC27255_01432

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ATCC 27255
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Eubacteriales > Oscillospiraceae > Ruminococcus

Accessions

  • Primary accession
    A0A2N0UL18

Proteomes

Subcellular Location

Keywords

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-73Methylguanine DNA methyltransferase ribonuclease-like
Domain78-162Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding

Sequence similarities

Belongs to the MGMT family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    174
  • Mass (Da)
    19,014
  • Last updated
    2018-04-25 v1
  • Checksum
    3632194581519EC4
MIFTDYYNSPIGGITMASEGEGLCGLWFDGQKYFASTVKGETEDKSLPVFEKTKKWLDIYFSGEEPDFTPKLSLNGSKFRKAVWDILLTIPYGSVMTYGEIARIMAKQSGIAKMSAQAVGGAVGHNPVSIIVPCHRVVGTNGNLTGYAGGIDKKISLLKNEKTDISKMFVLKKN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NNSR01000069
EMBL· GenBank· DDBJ
PKD27671.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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