A0A2L2XSK2 · A0A2L2XSK2_9CHRO

  • Protein
    Acireductone dioxygenase
  • Gene
    mtnD
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Fe2+ (UniProtKB | Rhea| CHEBI:29033 )

Note: Binds 1 Fe2+ cation per monomer.
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Note: Binds 1 nickel ion per monomer.

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site99Fe2+ (UniProtKB | ChEBI)
Binding site99Ni2+ (UniProtKB | ChEBI)
Binding site101Fe2+ (UniProtKB | ChEBI)
Binding site101Ni2+ (UniProtKB | ChEBI)
Site104May play a role in transmitting local conformational changes
Binding site105Fe2+ (UniProtKB | ChEBI)
Binding site105Ni2+ (UniProtKB | ChEBI)
Site107Important to generate the dianion
Binding site144Fe2+ (UniProtKB | ChEBI)
Binding site144Ni2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionacireductone dioxygenase (Ni2+-requiring) activity
Molecular Functionacireductone dioxygenase [iron(II)-requiring] activity
Molecular Functioniron ion binding
Molecular Functionnickel cation binding
Biological ProcessL-methionine salvage from methylthioadenosine
Biological ProcessL-methionine salvage from S-adenosylmethionine

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Acireductone dioxygenase
  • Alternative names
    • 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
      (DHK-MTPene dioxygenase
      )
    • Acireductone dioxygenase (Fe(2+)-requiring)
      (ARD'
      ; Fe-ARD
      ) (EC:1.13.11.54
      ) . EC:1.13.11.54 (UniProtKB | ENZYME | Rhea)
    • Acireductone dioxygenase (Ni(2+)-requiring)
      (ARD
      ; Ni-ARD
      ) (EC:1.13.11.53
      ) . EC:1.13.11.53 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      mtnD
    • ORF names
      N0824_02867

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 0824
  • Taxonomic lineage
    Bacteria > Cyanobacteriota > Cyanophyceae > Oscillatoriophycideae > Chroococcales > Microcystaceae > Microcystis

Accessions

  • Primary accession
    A0A2L2XSK2

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Sequence similarities

Belongs to the acireductone dioxygenase (ARD) family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    183
  • Mass (Da)
    20,888
  • Last updated
    2018-04-25 v1
  • Checksum
    F785F895547294D1
MAILRLENGTTYTQLADISLELAKLNVTLNYWPIENEATRQLLKQASLTDEEKEIVLTSLDGYFEQLKQEAGYQARDLIVLHPEIANLDTLLAKFERCHTHADDEVRYIIDGEGVFGFVFPDGSQGELTIQPQEYINVPAHSEHWFHLTASKRVKAVRYFTTTAGWVPEYTETVIRFPSLTVV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BDSM01000043
EMBL· GenBank· DDBJ
GBF54998.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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