A0A2L2K7I0 · A0A2L2K7I0_9VIBR
- Protein3-methyl-2-oxobutanoate hydroxymethyltransferase
- GenepanB
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids264 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.
Catalytic activity
- 3-methyl-2-oxobutanoate + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H2O = 2-dehydropantoate + (6S)-5,6,7,8-tetrahydrofolate
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 45 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 45-46 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: DS | ||||||
Binding site | 84 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 84 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 112 | 3-methyl-2-oxobutanoate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 114 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Active site | 181 | Proton acceptor | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 3-methyl-2-oxobutanoate hydroxymethyltransferase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | pantothenate biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name3-methyl-2-oxobutanoate hydroxymethyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Vibrionales > Vibrionaceae > Vibrio > Vibrio diabolicus subgroup
Accessions
- Primary accessionA0A2L2K7I0
Proteomes
Subcellular Location
Interaction
Subunit
Homodecamer; pentamer of dimers.
Structure
Sequence
- Sequence statusComplete
- Length264
- Mass (Da)28,748
- Last updated2018-06-20 v1
- ChecksumBE17172469268F3F
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP069195 EMBL· GenBank· DDBJ | QRG83393.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
PKPZ01000012 EMBL· GenBank· DDBJ | RPB38550.1 EMBL· GenBank· DDBJ | Genomic DNA |