A0A2L1L6K7 · A0A2L1L6K7_9INFA
- ProteinRNA-directed RNA polymerase catalytic subunit
- GenePB1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids757 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copies vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.
RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.
Catalytic activity
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | host cell cytoplasm | |
Cellular Component | host cell nucleus | |
Molecular Function | nucleotide binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Biological Process | DNA-templated transcription | |
Biological Process | symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity | |
Biological Process | viral RNA genome replication | |
Biological Process | viral transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRNA-directed RNA polymerase catalytic subunit
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Negarnaviricota > Polyploviricotina > Insthoviricetes > Articulavirales > Orthomyxoviridae > Alphainfluenzavirus > Alphainfluenzavirus influenzae
Accessions
- Primary accessionA0A2L1L6K7
Proteomes
Subcellular Location
PTM/Processing
Post-translational modification
Phosphorylated by host PRKCA.
Keywords
- PTM
Interaction
Subunit
Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.
Structure
Family & Domains
Features
Showing features for compositional bias, region, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 51-65 | Polar residues | ||||
Sequence: EKGKWTTNSETGAPQ | ||||||
Region | 51-82 | Disordered | ||||
Sequence: EKGKWTTNSETGAPQLNPIDGPLPEDNEPSGY | ||||||
Motif | 187-195 | Nuclear localization signal | ||||
Sequence: RKRRVRDNM | ||||||
Motif | 203-216 | Nuclear localization signal | ||||
Sequence: RTIGKKKQRLNKKS | ||||||
Region | 249-256 | Promoter-binding site | ||||
Sequence: RGFVYFVE | ||||||
Domain | 286-483 | RdRp catalytic | ||||
Sequence: VRKMMTNSQDTELSFTITGDNTKWNENQNPRMFLAMITYITRDQPEWFRNVLSIAPIMFSNKMARLGKGYMFESKSMKLRTQIPAEMLASIDLKYFNESTRKKIEKIRPLLIDGTASLSPGMMMGMFNMLSTVLGVSILNLGQKRYTKTTYWWDGLQSSDDFALIVNAPNHEGIQAGVDRFYRTCKLVGINMSKKKSY |
Sequence similarities
Belongs to the influenza viruses polymerase PB1 family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length757
- Mass (Da)86,385
- Last updated2018-04-25 v1
- Checksum290D0269D8B89774
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 51-65 | Polar residues | ||||
Sequence: EKGKWTTNSETGAPQ |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MG964662 EMBL· GenBank· DDBJ | AVE28803.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG964814 EMBL· GenBank· DDBJ | AVE29031.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG964822 EMBL· GenBank· DDBJ | AVE29043.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG964854 EMBL· GenBank· DDBJ | AVE29091.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965078 EMBL· GenBank· DDBJ | AVE29427.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965110 EMBL· GenBank· DDBJ | AVE29475.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965126 EMBL· GenBank· DDBJ | AVE29499.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965142 EMBL· GenBank· DDBJ | AVE29523.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965158 EMBL· GenBank· DDBJ | AVE29547.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965198 EMBL· GenBank· DDBJ | AVE29607.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965214 EMBL· GenBank· DDBJ | AVE29631.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965222 EMBL· GenBank· DDBJ | AVE29643.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965262 EMBL· GenBank· DDBJ | AVE29703.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965270 EMBL· GenBank· DDBJ | AVE29715.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965294 EMBL· GenBank· DDBJ | AVE29751.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965334 EMBL· GenBank· DDBJ | AVE29811.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965342 EMBL· GenBank· DDBJ | AVE29823.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965350 EMBL· GenBank· DDBJ | AVE29835.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965374 EMBL· GenBank· DDBJ | AVE29871.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965390 EMBL· GenBank· DDBJ | AVE29895.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965430 EMBL· GenBank· DDBJ | AVE29955.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965454 EMBL· GenBank· DDBJ | AVE29991.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965510 EMBL· GenBank· DDBJ | AVE30075.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965526 EMBL· GenBank· DDBJ | AVE30099.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965566 EMBL· GenBank· DDBJ | AVE30159.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965830 EMBL· GenBank· DDBJ | AVE30555.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965838 EMBL· GenBank· DDBJ | AVE30567.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965854 EMBL· GenBank· DDBJ | AVE30591.1 EMBL· GenBank· DDBJ | Viral cRNA | ||
MG965862 EMBL· GenBank· DDBJ | AVE30603.1 EMBL· GenBank· DDBJ | Viral cRNA |