A0A2K9VSG9 · A0A2K9VSG9_9HYPO

  • Protein
    DNA topoisomerase I
  • Gene
    TOP1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentchromosome
Cellular Componentnucleolus
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Biological Processchromosome segregation
Biological ProcessDNA replication
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase I
  • EC number
  • Alternative names
    • DNA topoisomerase 1

Gene names

    • Name
      TOP1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • CBS 123664
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium

Accessions

  • Primary accession
    A0A2K9VSG9

Subcellular Location

Family & Domains

Features

Showing features for region, compositional bias, domain, coiled coil.

TypeIDPosition(s)Description
Region1-255Disordered
Compositional bias81-128Basic and acidic residues
Compositional bias143-196Basic and acidic residues
Compositional bias211-226Basic and acidic residues
Compositional bias235-255Basic and acidic residues
Region422-441Disordered
Compositional bias425-439Basic and acidic residues
Domain425-880DNA topoisomerase I eukaryotic-type
Coiled coil792-847

Sequence similarities

Belongs to the type IB topoisomerase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    910
  • Mass (Da)
    103,830
  • Last updated
    2018-04-25 v1
  • Checksum
    F431469A1894F6B4
MSSSDDDRPLAKANGHRQSPLTVSAAKISRADDNAMAQSVPKEAAKMAGLSVRNGPIEDTSMDIDEPAANGTTKRKSRNSITKVNYKDGESSDEGVPLAKRQKKQITKVAESDSDDEPIMKARGKKLPPSYKETALPESSDDDDEPLGVKLAQKKANIEKKAEKEAKAIRSQERAKKPAPKKAIKDESDDDVPLARSTVTKRQTNGTAKRKSNGVKKEESDSDAPISKKVRPAAAKKGAKAESKKASESDGEEEFEWWNAPKKEDDSIKWTTLEHNGVLFPPDYEPLPKNVKMLYDGQPVTLAPEVEEVATFWVAMMTAASIHHLENPVFRKNFFDDFKVYCDKYGVTDVQGKKVAIKSLDKCNFDKINDYWTKRVEQNKTKNMTKEEKEAAKAKKDALEAPFTHCTWDGRKEKVGNFRVEPPSLFRGRGEHPKTGKVKQRVQPEQITINIGKGAKIPEPPKGHQWKAVQHDQKATWLAMWQENINQNYKYVMLGADSAIKGQSDFKKFEKARELKKHIDKIRKDYTKELKSEIMADRQRATAMYFIDKLALRAGNEKDTENEADTVGCCSLKYEHVTLEPPNKVTFDFLGKDSIPYRETAVVDAQVFKNLKLFKKAPKTTGDDLFDRLTTSQLNKHLTNYMKGLTAKVFRTYNASFTMSTLLNKLGSDPRSRGTVAEKVKLYNDCNREVAILCNHKRTVGASHEQQMAKLGDRIKGLRYQQWRTKMMILDVDSSYKKKKGTAWFEKDEELNDEWIKEHQQFLLEEQRTKIQKKFEKDNEKRKADKERPLPEKELKERLQVVKEMEAKFKKENKSKKVEAEGRGATVDKFLKAVDKFDERIKTLELQAQDRDGNKEVALGTSKINYIDPRLTVVFSKKFDVPIEKFFSKTLRDKFRWAIKSVEDEDDWTF

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias81-128Basic and acidic residues
Compositional bias143-196Basic and acidic residues
Compositional bias211-226Basic and acidic residues
Compositional bias235-255Basic and acidic residues
Compositional bias425-439Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
KY952943
EMBL· GenBank· DDBJ
AUV65419.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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