A0A2K9VSG9 · A0A2K9VSG9_9HYPO
- ProteinDNA topoisomerase I
- GeneTOP1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids910 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
Catalytic activity
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | nucleolus | |
Molecular Function | DNA binding | |
Molecular Function | DNA topoisomerase type I (single strand cut, ATP-independent) activity | |
Biological Process | chromosome segregation | |
Biological Process | DNA replication | |
Biological Process | DNA topological change |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA topoisomerase I
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium
Accessions
- Primary accessionA0A2K9VSG9
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-255 | Disordered | ||||
Sequence: MSSSDDDRPLAKANGHRQSPLTVSAAKISRADDNAMAQSVPKEAAKMAGLSVRNGPIEDTSMDIDEPAANGTTKRKSRNSITKVNYKDGESSDEGVPLAKRQKKQITKVAESDSDDEPIMKARGKKLPPSYKETALPESSDDDDEPLGVKLAQKKANIEKKAEKEAKAIRSQERAKKPAPKKAIKDESDDDVPLARSTVTKRQTNGTAKRKSNGVKKEESDSDAPISKKVRPAAAKKGAKAESKKASESDGEEEF | ||||||
Compositional bias | 81-128 | Basic and acidic residues | ||||
Sequence: ITKVNYKDGESSDEGVPLAKRQKKQITKVAESDSDDEPIMKARGKKLP | ||||||
Compositional bias | 143-196 | Basic and acidic residues | ||||
Sequence: DDEPLGVKLAQKKANIEKKAEKEAKAIRSQERAKKPAPKKAIKDESDDDVPLAR | ||||||
Compositional bias | 211-226 | Basic and acidic residues | ||||
Sequence: KSNGVKKEESDSDAPI | ||||||
Compositional bias | 235-255 | Basic and acidic residues | ||||
Sequence: AKKGAKAESKKASESDGEEEF | ||||||
Region | 422-441 | Disordered | ||||
Sequence: PPSLFRGRGEHPKTGKVKQR | ||||||
Compositional bias | 425-439 | Basic and acidic residues | ||||
Sequence: LFRGRGEHPKTGKVK | ||||||
Domain | 425-880 | DNA topoisomerase I eukaryotic-type | ||||
Sequence: LFRGRGEHPKTGKVKQRVQPEQITINIGKGAKIPEPPKGHQWKAVQHDQKATWLAMWQENINQNYKYVMLGADSAIKGQSDFKKFEKARELKKHIDKIRKDYTKELKSEIMADRQRATAMYFIDKLALRAGNEKDTENEADTVGCCSLKYEHVTLEPPNKVTFDFLGKDSIPYRETAVVDAQVFKNLKLFKKAPKTTGDDLFDRLTTSQLNKHLTNYMKGLTAKVFRTYNASFTMSTLLNKLGSDPRSRGTVAEKVKLYNDCNREVAILCNHKRTVGASHEQQMAKLGDRIKGLRYQQWRTKMMILDVDSSYKKKKGTAWFEKDEELNDEWIKEHQQFLLEEQRTKIQKKFEKDNEKRKADKERPLPEKELKERLQVVKEMEAKFKKENKSKKVEAEGRGATVDKFLKAVDKFDERIKTLELQAQDRDGNKEVALGTSKINYIDPRLTVVFSKKFD | ||||||
Coiled coil | 792-847 | |||||
Sequence: EKELKERLQVVKEMEAKFKKENKSKKVEAEGRGATVDKFLKAVDKFDERIKTLELQ |
Sequence similarities
Belongs to the type IB topoisomerase family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length910
- Mass (Da)103,830
- Last updated2018-04-25 v1
- ChecksumF431469A1894F6B4
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 81-128 | Basic and acidic residues | ||||
Sequence: ITKVNYKDGESSDEGVPLAKRQKKQITKVAESDSDDEPIMKARGKKLP | ||||||
Compositional bias | 143-196 | Basic and acidic residues | ||||
Sequence: DDEPLGVKLAQKKANIEKKAEKEAKAIRSQERAKKPAPKKAIKDESDDDVPLAR | ||||||
Compositional bias | 211-226 | Basic and acidic residues | ||||
Sequence: KSNGVKKEESDSDAPI | ||||||
Compositional bias | 235-255 | Basic and acidic residues | ||||
Sequence: AKKGAKAESKKASESDGEEEF | ||||||
Compositional bias | 425-439 | Basic and acidic residues | ||||
Sequence: LFRGRGEHPKTGKVK |