A0A2K9RFZ9 · TPS6_VITAC
- ProteinClass I diterpene synthase TPS6, chloroplastic
- GeneTPS6
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids594 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the biosynthesis of labdane-type diterpenoid including cleroda-dienols, and peregrinol lactones and furan derivatives, dopaminergic diterpenoids that can bind to dopamine receptors in the human pituitary gland, have probably ability to lower prolactin levels, and are used to treat menstrual cycle disorders (e.g. premenstrual syndrome and mastodynia) (Probable). Terpene synthase the catalyzes the conversion of peregrinol diphosphate to labda-1316,14-dien-9-ol, and of syn-copalyl diphosophate to dehydroabietadiene and syn-isopimara-7,15-diene (PubMed:29315936).
Catalytic activity
- peregrinol diphosphate = labd-1316,14-diene-9-ol + diphosphateThis reaction proceeds in the forward direction.
- 9alpha-copalyl diphosphate = syn-isopimara-7,15-diene + diphosphateThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 3 Mg2+ ions per subunit.
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 330 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 330 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 334 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 334 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 474 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 477 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 482 | Mg2+ 3 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | Labd-13(16),14-diene-9-ol synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | syn-isopimara-7,15-diene synthase activity | |
Molecular Function | terpene synthase activity | |
Biological Process | gibberellin biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameClass I diterpene synthase TPS6, chloroplastic
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Lamiales > Lamiaceae > Viticoideae > Vitex
Accessions
- Primary accessionA0A2K9RFZ9
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000449312 | 1-594 | Class I diterpene synthase TPS6, chloroplastic | ||
Expression
Tissue specificity
Mostly expressed in trichomes of leaves and fruits.
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 330-334 | DDXXD motif | |||
Domain
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.
Sequence similarities
Belongs to the terpene synthase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length594
- Mass (Da)69,281
- Last updated2018-04-25 v1
- Checksum24C183A37ED574D9
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MG696753 EMBL· GenBank· DDBJ | AUT77125.1 EMBL· GenBank· DDBJ | mRNA |