A0A2K9PDT9 · A0A2K9PDT9_9ENTR

Function

function

Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Pathway

Carbohydrate acid metabolism; galactarate degradation; D-glycerate from galactarate: step 2/3.

Features

Showing features for active site, site, binding site.

Type
IDPosition(s)Description
Active site50Proton acceptor
Site75Transition state stabilizer
Site89Increases basicity of active site His
Binding site151substrate
Binding site153Mg2+ (UniProtKB | ChEBI)
Binding site178substrate
Binding site179Mg2+ (UniProtKB | ChEBI)
Binding site179substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function2-dehydro-3-deoxyglucarate aldolase activity
Molecular Functionmagnesium ion binding
Biological ProcessD-glucarate catabolic process
Biological Processgalactarate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    5-keto-4-deoxy-D-glucarate aldolase
  • EC number
  • Short names
    KDGluc aldolase
    ; KDGlucA
  • Alternative names
    • 2-dehydro-3-deoxy-D-glucarate aldolase
    • 2-keto-3-deoxy-D-glucarate aldolase
    • 5-dehydro-4-deoxy-D-glucarate aldolase
    • Alpha-keto-beta-deoxy-D-glucarate aldolase

Gene names

    • Name
      garL
    • ORF names
      WM46_16435

Organism names

  • Taxonomic identifier
  • Strain
    • CFNIH2
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Citrobacter > Citrobacter freundii complex

Accessions

  • Primary accession
    A0A2K9PDT9

Proteomes

Subcellular Location

Interaction

Subunit

Homohexamer; trimer of dimers.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain20-245HpcH/HpaI aldolase/citrate lyase

Sequence similarities

Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    256
  • Mass (Da)
    27,362
  • Last updated
    2018-04-25 v1
  • MD5 Checksum
    82C942DC0AB7E12611F30FEB8B8FA510
MNNDIFPNKFKAALAAHQIQIGCWSALASPISTEVLGLAGFDWLVLDGEHAPNDVSTFIPQLMALKGSSSAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVETEEEAVNAVASTRYPPEGIRGVSVSHRANMFGTVPDYFAQSNKNITIIVQIESQQGVDNVDAIAATEGVDGIFVGPSDLAAALGHLGNASHPEVQKAIQHIFARAKAHGKPSGILAPVDADARRYLEWGATFVAVGSDLGVFRSATQKLADSFKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP025757
EMBL· GenBank· DDBJ
AUO66201.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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