A0A2K8HH98 · A0A2K8HH98_9CAUD

Function

function

The terminase large subunit acts as an ATP driven molecular motor necessary for viral DNA translocation into empty capsids and as an endonuclease that cuts the viral genome to initiate and to end a packaging reaction. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (packaging sequence), and a large terminase subunit possessing endonucleolytic and ATPase activities. Both terminase subunits heterooligomerize and are docked on the portal protein to form the packaging machine. The terminase large subunit exhibits endonuclease activity and cleaves the viral genome concatemer. Once the capsid is packaged with the DNA, the terminase complex is substituted by the tail.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: ATPase activity requires 1 Mg2+ ion per subunit. Nuclease activity probably requires 2 Mg2+ ions per subunit.

Features

Showing features for active site, binding site.

143850100150200250300350400
TypeIDPosition(s)Description
Active site155For ATPase activity
Binding site286Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site286Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site342Mg2+ 2 (UniProtKB | ChEBI); catalytic; for nuclease activity
Binding site418Mg2+ 1 (UniProtKB | ChEBI); catalytic; for nuclease activity

GO annotations

AspectTerm
Cellular Componentviral terminase, large subunit
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular Functionendonuclease activity
Molecular Functionmetal ion binding
Biological Processchromosome organization
Biological Processviral DNA genome packaging

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Terminase, large subunit
  • Alternative names
    • DNA-packaging protein

Including 2 domains:

  • Recommended name
    ATPase
  • EC number
  • Recommended name
    Endonuclease
  • EC number

Gene names

    • ORF names
      P27_0148

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Viruses > Duplodnaviria > Heunggongvirae > Uroviricota > Caudoviricetes > Demerecviridae > Markadamsvirinae > Tequintavirus > Tequintavirus chee24

Accessions

  • Primary accession
    A0A2K8HH98

Proteomes

Subcellular Location

Interaction

Subunit

Interacts with the terminase small subunit; the active complex is probably heterooligomeric. Interacts with the portal protein.

Structure

3D structure databases

Family & Domains

Features

Showing features for motif, domain.

TypeIDPosition(s)Description
Motif62-68Walker A motif
Motif150-155Walker B motif
Domain284-431Terminase large subunit gp17-like C-terminal

Domain

The N-terminus contains an ATPase domain. The C-terminus contains an endonuclease domain.

Sequence similarities

Belongs to the Tequatrovirus large terminase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    438
  • Mass (Da)
    49,717
  • Last updated
    2018-04-25 v1
  • Checksum
    2BF5B121DEC69102
MEVSRPYVNTVDVIDFGIDKRFFRLPVSGILAQEGITPNGPQIAIINALEDPRHRFVTACVSRRVGKSFIAYTLGFLKLLEPNVKVLVVAPNYSLANIGWSQIRGLIKKYGLQTERENAKDKEIELANGSLFKLASAAQADSAVGRSYDFIIFDEAAISDVGGDAFRVQLRPTLDKPNSKALFISTPRGGNWFKEFYAYGFDDTLPNWVSIHGTYRDNPRADLNDIEEARRTVSKNYFRQEYEADFSVFEGQIFDTFNAIDHVKDLKGMRHFFKDDEAFETLLGIDVGYRDPTAVLTIKYHYDTDTYYVLEEYQQAEKTTAQHAAYIQHCIDRYKVDRIFVDSAAAQFRQDLAYEHEIASAPAKKSVLDGLACLQALFQQGKIIVDASCSSLIHALQNYKWDFQEGEEKLSREKPRHDANSHLCDALRYGIYSISRGK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MF431731
EMBL· GenBank· DDBJ
ASU01753.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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