A0A2K6CMY7 · A0A2K6CMY7_MACNE

Function

function

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

Catalytic activity

Features

Showing features for site, binding site.

TypeIDPosition(s)Description
Site46Crucial for catalytic activity
Binding site112-125acetyl-CoA (UniProtKB | ChEBI)
Binding site148-157acetyl-CoA (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentaxon
Cellular Componentclathrin-coated pit
Cellular Componentcytoplasm
Cellular Componentfocal adhesion
Cellular Componentmicrotubule
Cellular Componentspindle
Molecular Functiontubulin N-acetyltransferase activity
Biological Processneuron development
Biological Processregulation of microtubule cytoskeleton organization

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-tubulin N-acetyltransferase 1
  • EC number
  • Short names
    Alpha-TAT
    ; Alpha-TAT1
    ; TAT
  • Alternative names
    • Acetyltransferase mec-17 homolog

Gene names

    • Name
      ATAT1
    • Synonyms
      MEC17

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Cercopithecinae > Macaca

Accessions

  • Primary accession
    A0A2K6CMY7

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue44N6-acetyllysine; by autocatalysis
Modified residue134N6-acetyllysine; by autocatalysis

Post-translational modification

Autoacetylation strongly increases tubulin acetylation.

Keywords

Expression

Gene expression databases

Interaction

Subunit

Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain1-178N-acetyltransferase
Region184-227Disordered
Compositional bias212-226Basic and acidic residues
Region240-272Disordered
Compositional bias243-258Pro residues
Region294-390Disordered
Compositional bias325-344Polar residues

Sequence similarities

Belongs to the acetyltransferase ATAT1 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    411
  • Mass (Da)
    45,756
  • Last updated
    2018-03-28 v1
  • Checksum
    B0F8AD4F060E1168
MWLTRPFCFLTITLREEGVYHLESVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSGRPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPTAPARKLPPKRAEGDIKPYSSSDREFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPPHPTARLLLAADPGGSPAQRRRTRGTPPGLVAQSCCYSRHGGVNSSSPNTGNQDSKQGEQETKNRSASEEQALSQDGSGEKPVHTAPPQAPAPAPPAQSWTVGGDILNARFIRNLQERRSTRPW

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2K6CN54A0A2K6CN54_MACNEATAT1413
A0A2K6CN16A0A2K6CN16_MACNEATAT1333
A0A2K6CN27A0A2K6CN27_MACNEATAT1310
A0A2K6CN53A0A2K6CN53_MACNEATAT1179

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias212-226Basic and acidic residues
Compositional bias243-258Pro residues
Compositional bias325-344Polar residues

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

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