A0A2K5I6P9 · A0A2K5I6P9_COLAP

Function

Catalytic activity

  • 24,25-dihydrolanosterol + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 4 H+ + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • 24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-hydroxy-24,25-dihydrolanosterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • 32-hydroxy-24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-oxo-24,25-dihydrolanosterol + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • 32-hydroxylanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-oxolanosterol + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • 32-oxo-24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • 32-oxolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • a 14alpha-formyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a Delta14 steroid + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • a 14alpha-hydroxymethyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a 14alpha-formyl steroid + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • a 14alpha-methyl steroid + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = a Delta14 steroid + formate + 4 H+ + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
    EC:1.14.14.154 (UniProtKB | ENZYME | Rhea)
  • a 14alpha-methyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a 14alpha-hydroxymethyl steroid + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
  • lanosterol + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 4 H+ + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.
    EC:1.14.14.154 (UniProtKB | ENZYME | Rhea)
  • lanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-hydroxylanosterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]
    This reaction proceeds in the forward direction.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site455Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
Biological Processcholesterol metabolic process
Biological Processorganic hydroxy compound biosynthetic process
Biological Processsmall molecule biosynthetic process
Biological Processsteroid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Lanosterol 14-alpha demethylase
  • EC number
  • Alternative names
    • CYPLI
    • Cytochrome P450 51A1
    • Cytochrome P450-14DM
    • Cytochrome P450LI

Gene names

    • Name
      CYP51A1

Organism names

Accessions

  • Primary accession
    A0A2K5I6P9

Proteomes

Organism-specific databases

Subcellular Location

Endoplasmic reticulum membrane
; Single-pass membrane protein
Microsome membrane
; Single-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane31-50Helical

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-21
ChainPRO_501436112422-509Lanosterol 14-alpha demethylase

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    509
  • Mass (Da)
    57,288
  • Last updated
    2018-03-28 v1
  • Checksum
    C81CA9A5F803A67A
MAAAAGMMLLGLLQAGGSVLGQAMEKVTGGNLLSMLLIACAFTLSLVYLFRLAAGHLVQLPAGAKSPPYIFSPIPFLGHAIAFGKSPVEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLKSGLNIAHFKQHVSIIEKETKEYFQSWGESGEKNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCYLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMIMMRMARTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPENPVIRYKRRSK

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A2K5I6T4A0A2K5I6T4_COLAPCYP51A1507

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

Disclaimer

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