A0A2K5I6P9 · A0A2K5I6P9_COLAP
- ProteinLanosterol 14-alpha demethylase
- GeneCYP51A1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids509 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
Catalytic activity
- 24,25-dihydrolanosterol + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 4 H+ + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
CHEBI:28113 + 3 CHEBI:15379 + 3 RHEA-COMP:11964 = CHEBI:78904 + CHEBI:15740 + 4 CHEBI:15378 + 4 CHEBI:15377 + 3 RHEA-COMP:11965 - 24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-hydroxy-24,25-dihydrolanosterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-hydroxy-24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-oxo-24,25-dihydrolanosterol + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-hydroxylanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-oxolanosterol + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-oxo-24,25-dihydrolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- 32-oxolanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- a 14alpha-formyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a Delta14 steroid + formate + 2 H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- a 14alpha-hydroxymethyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a 14alpha-formyl steroid + H+ + 2 H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- a 14alpha-methyl steroid + O2 + reduced [NADPH--hemoprotein reductase] = a 14alpha-hydroxymethyl steroid + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
- lanosterol + O2 + reduced [NADPH--hemoprotein reductase] = 32-hydroxylanosterol + H+ + H2O + oxidized [NADPH--hemoprotein reductase]This reaction proceeds in the forward direction.
Cofactor
Pathway
Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | monooxygenase activity | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | |
Biological Process | cholesterol metabolic process | |
Biological Process | organic hydroxy compound biosynthetic process | |
Biological Process | small molecule biosynthetic process | |
Biological Process | steroid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLanosterol 14-alpha demethylase
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Primates > Haplorrhini > Catarrhini > Cercopithecidae > Colobinae > Colobus
Accessions
- Primary accessionA0A2K5I6P9
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Microsome membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 31-50 | Helical | ||||
Sequence: NLLSMLLIACAFTLSLVYLF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-21 | |||||
Sequence: MAAAAGMMLLGLLQAGGSVLG | ||||||
Chain | PRO_5014361124 | 22-509 | Lanosterol 14-alpha demethylase | |||
Sequence: QAMEKVTGGNLLSMLLIACAFTLSLVYLFRLAAGHLVQLPAGAKSPPYIFSPIPFLGHAIAFGKSPVEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNEDLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKMLKSGLNIAHFKQHVSIIEKETKEYFQSWGESGEKNVFEALSELIILTASHCLHGKEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKTLQEKCYLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMIMMRMARTPQTVAGYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYLQDNPASGEKFAYVPFGAGRHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTMIHTPENPVIRYKRRSK |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length509
- Mass (Da)57,288
- Last updated2018-03-28 v1
- ChecksumC81CA9A5F803A67A
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A2K5I6T4 | A0A2K5I6T4_COLAP | CYP51A1 | 507 |
Keywords
- Technical term