A0A2K2H9A0 · A0A2K2H9A0_9BACT
- ProteinPhosphoenolpyruvate-protein phosphotransferase
- GeneptsP
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids594 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).
Catalytic activity
- L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-L-histidyl-[protein] + pyruvate
Cofactor
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 201 | Tele-phosphohistidine intermediate | ||||
Sequence: H | ||||||
Binding site | 308 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 344 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 443 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 466-467 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: ND | ||||||
Binding site | 467 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 477 | phosphoenolpyruvate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Active site | 514 | Proton donor | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | membrane | |
Molecular Function | kinase activity | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoenolpyruvate-protein phosphotransferase activity | |
Biological Process | phosphoenolpyruvate-dependent sugar phosphotransferase system |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate-protein phosphotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermodesulfobacteriota > Desulfuromonadia > Desulfuromonadales > Geothermobacteraceae > Geothermobacter
Accessions
- Primary accessionA0A2K2H9A0
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 12-32 | Helical | ||||
Sequence: TFLVGIGVSPGIAIGATHLVN |
Keywords
- Cellular component
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 16-138 | Phosphotransferase system enzyme I N-terminal | ||||
Sequence: GIGVSPGIAIGATHLVNRARMAALERRIRKSDVDVEIAAFEEAVELSRRQLHEVKRRVADRHLAEHLYIIDTHLLILEDQMLHAETRRGIREDLLNAEGALKRALDQFRSVFESIEDEYLRDR | ||||||
Domain | 165-237 | PEP-utilising enzyme mobile | ||||
Sequence: DRKVVVVAHDLSPADTMQIDKDKIIGFVTDVGGRTSHTAILARSQGIPAVVGLENATALIPGGTPLIVDGSSG | ||||||
Domain | 268-553 | PEP-utilising enzyme C-terminal | ||||
Sequence: YRSLPAETPDGHRLVLRCNVELPSEVPHALAQGAEGVGLFRSEFLYLGRQFPPDEEEQFDAYREIVTAMAPHEVTIRTLDVGGDKFVPEINLSDEVNPALGLRGVRFSLADRQLLKIQLRAILRVSALGPVRVMFPMISGVSEIRACKSILHQAMAELSREGIDFDGRIPVGIMIETPSAALIADKLAREVDFFSVGTNDLIQYCLAVDRSNEHVAYLYEPLHPAILRALQMICRAARQAGIKVAMCGEMAAEPLYLPILIGFGFGELSMNAPCIPRVKRVVRQIR |
Sequence similarities
Belongs to the PEP-utilizing enzyme family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length594
- Mass (Da)65,965
- Last updated2018-03-28 v1
- ChecksumF7259FC7EE560C97