A0A2K1K4G8 · A0A2K1K4G8_PHYPA
- ProteinATP-dependent 6-phosphofructokinase
- GenePFK
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids622 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 184 | ATP (UniProtKB | ChEBI) | |||
Binding site | 247-248 | ATP (UniProtKB | ChEBI) | |||
Binding site | 272-275 | ATP (UniProtKB | ChEBI) | |||
Binding site | 273 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Site | 274 | Important for substrate specificity; cannot use PPi as phosphoryl donor | |||
Binding site | 301-303 | substrate | |||
Active site | 303 | Proton acceptor | |||
Binding site | 346-348 | substrate | |||
Binding site | 402 | substrate | |||
Binding site | 460-463 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Bryophyta > Bryophytina > Bryopsida > Funariidae > Funariales > Funariaceae > Physcomitrium
Accessions
- Primary accessionA0A2K1K4G8
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Proteomic databases
Interaction
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 177-484 | Phosphofructokinase | |||
Region | 547-622 | Disordered | |||
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length622
- Mass (Da)67,884
- Last updated2018-03-28 v1
- ChecksumBD7E87238D9E8B90
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A7I4EJZ1 | A0A7I4EJZ1_PHYPA | 460 | |||
A0A7I4EK51 | A0A7I4EK51_PHYPA | 444 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ABEU02000009 EMBL· GenBank· DDBJ | PNR48658.1 EMBL· GenBank· DDBJ | Genomic DNA |