A0A2K1J0I5 · A0A2K1J0I5_PHYPA
- ProteinProbable ATP-dependent 6-phosphofructokinase
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids635 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H+
Cofactor
Activity regulation
Allosterically activated by fructose 2,6-bisphosphate.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 176-177 | ATP (UniProtKB | ChEBI) | ||||
Sequence: KD | ||||||
Site | 207 | Important for substrate specificity; cannot use PPi as phosphoryl donor | ||||
Sequence: G | ||||||
Binding site | 234-236 | substrate | ||||
Sequence: TID | ||||||
Active site | 236 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 273-274 | substrate; ligand shared between dimeric partners | ||||
Sequence: KY | ||||||
Binding site | 281-283 | substrate | ||||
Sequence: MGR | ||||||
Binding site | 342 | substrate | ||||
Sequence: E | ||||||
Binding site | 448-451 | substrate | ||||
Sequence: YQAR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | diphosphate-fructose-6-phosphate 1-phosphotransferase activity | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process | |
Biological Process | photosynthesis | |
Biological Process | response to glucose |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable ATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Bryophyta > Bryophytina > Bryopsida > Funariidae > Funariales > Funariaceae > Physcomitrium
Accessions
- Primary accessionA0A2K1J0I5
Proteomes
Genome annotation databases
Subcellular Location
PTM/Processing
Proteomic databases
Interaction
Subunit
Tetramer of two alpha (regulatory) and two beta (catalytic) chains.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 104-471 | Phosphofructokinase | ||||
Sequence: RIGVAFCGRQSPGGHNVITGLFDALKSQNANSILIGFIGGTEGIFSQRTVELTNDMLKDFRNQGGYDLLGRTKDQIKSYQQIKDAKAACEALKLDVLVLIGGCGTNSDAAQLAEFFLESGCTTKVVGIPVTIDGDLKNQFVETNVGFDTTCKVYSQLISNICTDALSAEKYYYFIRLMGRQASHVSLECTLQSQSNMVLLGEEVANSKMTLFDVTMQICDAIQARGEQGKNYGVVLLPEGLIERIPEFRALLQEINTLRKQDEDIGVDNISSRLTLWAAALYDFLPIFIKKQLLLDRESDGSVQLSQIETEKLLAQLVDTEMIRRTKTGLYEGKKFNAICHFFGYQARGSLPSNFDCNYAYTLGQTAY |
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'Long' sub-subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length635
- Mass (Da)69,347
- Last updated2018-03-28 v1
- Checksum4EBD12C0C3E68D69
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
ABEU02000018 EMBL· GenBank· DDBJ | PNR35038.1 EMBL· GenBank· DDBJ | Genomic DNA |