A0A2J9KLF8 · A0A2J9KLF8_HELPX

Function

function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site17[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site21[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site24[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site61[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site154[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionbiotin synthase activity
Molecular Functioniron ion binding
Biological Processbiotin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Biotin synthase
  • EC number

Gene names

    • Name
      bioB
    • ORF names
      C2840_07305
      , C2842_07300
      , SE88_07235

Organism names

  • Taxonomic identifier
  • Strains
    • 26695-1MET
    • 26695-dR
    • 26695-dRdM2
  • Taxonomic lineage
    Bacteria > Campylobacterota > Epsilonproteobacteria > Campylobacterales > Helicobacteraceae > Helicobacter

Accessions

  • Primary accession
    A0A2J9KLF8

Proteomes

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-228Radical SAM core

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    282
  • Mass (Da)
    31,475
  • Last updated
    2018-03-28 v1
  • MD5 Checksum
    0EC16BCAB5FFF36635C23846D425639F
MQEIFLCSISNVRSGDCKEDCAYCTQSSHHQGAIKRYKFKDEKVVLQEARALRQLGALGFCLVTSGRELDDEKCEYIAKLAKAINQEELGLHLIACCGRADLEQLEFLRDAGIHSYNHNLETSQNFFPKICSTHTWEERFITCENALRAGLGLCSGGIFGLNESWEDRIEMLRALASLSPHTTPINFFIKNPVLPIDAETLSADEALECVLLAKEFLPNARLMVAGGREVVFKDNDKKEAKLFEYGINAVVLGDYLTTKGKAPKKDIEKLLSYGLTMATSCH

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP010436
EMBL· GenBank· DDBJ
AJF11160.1
EMBL· GenBank· DDBJ
Genomic DNA
CP026326
EMBL· GenBank· DDBJ
AUV78515.1
EMBL· GenBank· DDBJ
Genomic DNA
CP026324
EMBL· GenBank· DDBJ
AUV80022.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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