A0A2J0SY25 · A0A2J0SY25_STEMA

Function

function

Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site230substrate
Binding site259substrate
Binding site288substrate
Binding site324substrate
Binding site344-346substrate
Binding site385-388substrate
Binding site424substrate
Binding site428Zn2+ (UniProtKB | ChEBI)
Binding site451substrate
Binding site492Zn2+ (UniProtKB | ChEBI)
Binding site572[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site575[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site580[4Fe-4S] cluster (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functioncarbon-carbon lyase activity
Molecular Functionzinc ion binding
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Phosphomethylpyrimidine synthase
  • EC number
  • Alternative names
    • Hydroxymethylpyrimidine phosphate synthase
      (HMP-P synthase
      ; HMP-phosphate synthase
      ; HMPP synthase
      )
    • Thiamine biosynthesis protein ThiC

Gene names

    • Name
      thiC
    • ORF names
      B9Y57_03725
      , D7Y33_18895

Organism names

Accessions

  • Primary accession
    A0A2J0SY25

Proteomes

Subcellular Location

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for compositional bias, region, domain.

TypeIDPosition(s)Description
Compositional bias1-21Polar residues
Region1-36Disordered
Domain22-99ThiC-associated

Sequence similarities

Belongs to the ThiC family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    614
  • Mass (Da)
    68,237
  • Last updated
    2018-03-28 v1
  • Checksum
    A1B60D14640857FE
MNAQLSALQQQAQQLSESVTRPIPGSRKIHVPGSRPDLQVPMREIALTRTPTLFGGEENAPVTVYDTSGPYTDPQARVDLSAGLPALRRAWIEERGDTEQLDGLSSSFGRDREHDPKLDAVRFPARSLPRRARSGANVTQMHYARRGIITPEMEFVAIRENQRLDAIRDAGLLQQHPGEAFGASIQKIITPEFVRDEIARGRAVLPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGIAEEVEKLVWAIRWGGDTVMDLSTGKHIHETREWIIRNSPVAIGTVPIYQALEKVDGRAEALTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIMKAYDVTFSLGDGLRPGCIADANDAAQFGELETLGELTKIAWKHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPNRQDVRDGIMAYRIAAHAADLAKGHPGAQVRDNALSKARFEFRWEDQFHLGLDPEKAKEFHDETLPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAEAGMKEKSAEFRAAGAEVYHQG

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-21Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
RAUE01000031
EMBL· GenBank· DDBJ
MBA0313054.1
EMBL· GenBank· DDBJ
Genomic DNA
NEQP01000003
EMBL· GenBank· DDBJ
PJL03037.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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