A0A2I0W702 · A0A2I0W702_9ASPA
- ProteinGlutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial
- GeneGATA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids548 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in chloroplasts and mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Miscellaneous
This protein may be expected to contain an N-terminal transit peptide but none has been predicted.
Catalytic activity
- ATP + H2O + L-glutamine + L-glutamyl-tRNA(Gln) = ADP + H+ + L-glutamate + L-glutaminyl-tRNA(Gln) + phosphate
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 128 | Charge relay system | ||||
Sequence: K | ||||||
Active site | 203 | Charge relay system | ||||
Sequence: S | ||||||
Active site | 227 | Acyl-ester intermediate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast stroma | |
Cellular Component | glutamyl-tRNA(Gln) amidotransferase complex | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Molecular Function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | |
Molecular Function | transferase activity | |
Biological Process | glutaminyl-tRNAGln biosynthesis via transamidation | |
Biological Process | mitochondrial translation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit A, chloroplastic/mitochondrial
- EC number
- Short namesGlu-AdT subunit A
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Asparagales > Orchidaceae > Epidendroideae > Malaxideae > Dendrobiinae > Dendrobium
Accessions
- Primary accessionA0A2I0W702
Proteomes
Subcellular Location
Interaction
Subunit
Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 22-53 | Disordered | ||||
Sequence: SRSTSPRQLLHGADTASATLSSPPSNTVASGV | ||||||
Domain | 77-525 | Amidase | ||||
Sequence: GEYLDRLHKVEPQIRSFLHVSESAMKEAEEVDRMVAAGRELPPLAGVLVGVKDNICTVDMPSTGGSKILKGYRPAFDATAVRRLREAGAIVVGKTNLDEFGMGSSTEGSGFQVTANPWDLSRVPGGSSGGSAAAVSARQCVVSLGSDTGGSVRQPASFCGVVGMKPTYGRVSRFGLMAYASSLDVIGCFGSSIADVAILLNVISGHDRLDSTSSKRDVPDFANELVSLNNLDSKPLKGLKVGLIRETIGEGVDAGVVSAVQAAASHLEHLGCVLNEISLPSFSLGLPAYYILASSEASSNLSRYDGLRYGNQVGNGELSSIYGHSRANGFGDEVKMRILMGTYALSAGYYDAYYKRAQQVRTLIQKCFRDAMNDNDILISPAAPSAAYKIGEKTNNPLAMYAGDIMTVNVNLAGLPALVVPCGFVDGGPAGLPVGLQIIAAAFCEDTLL |
Sequence similarities
Belongs to the amidase family. GatA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length548
- Mass (Da)57,780
- Last updated2018-02-28 v1
- Checksum232BEF91756D5C0F
Keywords
- Technical term