A0A2I0KC25 · A0A2I0KC25_PUNGR
- ProteinATP-dependent 6-phosphofructokinase
- GenePFK
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids487 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity
- beta-D-fructose 6-phosphate + ATP = beta-D-fructose 1,6-bisphosphate + ADP + H+
Cofactor
Activity regulation
Allosterically activated by AMP.
Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 101 | ATP (UniProtKB | ChEBI) | |||
Binding site | 164-165 | ATP (UniProtKB | ChEBI) | |||
Binding site | 189-192 | ATP (UniProtKB | ChEBI) | |||
Binding site | 190 | Mg2+ (UniProtKB | ChEBI); catalytic | |||
Site | 191 | Important for substrate specificity; cannot use PPi as phosphoryl donor | |||
Binding site | 218-220 | substrate | |||
Active site | 220 | Proton acceptor | |||
Binding site | 263-265 | substrate | |||
Binding site | 319 | substrate | |||
Binding site | 375-378 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 6-phosphofructokinase activity | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Biological Process | fructose 6-phosphate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent 6-phosphofructokinase
- EC number
- Short namesATP-PFK ; Phosphofructokinase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Myrtales > Lythraceae > Punica
Accessions
- Primary accessionA0A2I0KC25
Proteomes
Subcellular Location
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 95-399 | Phosphofructokinase | |||
Sequence similarities
Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length487
- Mass (Da)53,550
- Last updated2018-02-28 v1
- Checksum832A85D8C47F5BA7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
PGOL01000690 EMBL· GenBank· DDBJ | PKI66089.1 EMBL· GenBank· DDBJ | Genomic DNA |