A0A2H9SV32 · A0A2H9SV32_9BACT

  • Protein
    DNA topoisomerase 1
  • Gene
    topA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

Type
IDPosition(s)Description
Site33Interaction with DNA
Site138Interaction with DNA
Site139Interaction with DNA
Site142Interaction with DNA
Site147Interaction with DNA
Active site314O-(5'-phospho-DNA)-tyrosine intermediate
Site316Interaction with DNA
Site508Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      CK425_02690

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BC.030
  • Taxonomic lineage
    Bacteria > Chlamydiota > Chlamydiia > Parachlamydiales > Parachlamydiaceae > Parachlamydia

Accessions

  • Primary accession
    A0A2H9SV32

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain3-113Toprim
Region166-171Interaction with DNA
Region757-791Disordered
Domain784-861DM2

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    863
  • Mass (Da)
    97,380
  • Last updated
    2018-02-28 v1
  • Checksum
    AEC5467F39A39792
MGKALIIVESPAKIKTLRKFLGANYVFESSIGHIRDLPEKEFGIDIENDFEPKYVVMPDKEKVIANLRKAAKECDIVYLSPDPDREGEAIAWHITQILPPSTKIKRVSFNSITKEAVVKALESPRGIDIALVNAQQARRLLDRIVGYKISPILNRRIQRGKEGFVSAGRVQSVALKLVVDREKEIEIFKPVEYWNLGAILKTEEDPKNFRANLHLVGGKRIEKELIEGKEVILIDTKEKADEILARLKGQSFQVVKVEKKEKKRNPVPPFITSTLQQEASRHYGFSSAKTMNIAQTLYEGVELGNEGAEGLITYMRTDSVRIAPEALEEVRSLILKQFGKDFLPADPKNYATSKSAQDAHEAIRPANLMHAPEAIKDYLSREQFLLYQLIWKRFIASQMMPAIYDTVSADIAAGKEFILRATGSIMKFQGFLAIYEEKEDDEDKTDEARMLPQLTEGQFLKLLELTSEQAFTRPPPRFTEASLVKELEKCRIGRPSTYAAIMNKIQSRDYTTKENGRLKPTELGIVIAQMLESNFQEIMNIGFTASMEDGLEAIAENQQDWKGLIRDFWVKFLPTLDEAEKSAFVPKVMTDIDCPTCGSKLQKIWFKSKYFYGCSRYPDCNFTAPLEEIAFNKEDYASDFDWEQKCPECQSDMKVRHGRFGAFLGCTRYPDCKGIINIPKKGETIIPAEDMPHCVAIGCTGHMVARKSRFGKTFYSCSTFPECDVIVNDLAQLSEKYPEHPRTAYIKKAGKGKKGAVKKTVAKKSTATKAAPKKAAVKKTATPRQQPQQQLSAELSQVVGAAEMSRGEVMKKVWEYIHAHHLQDTTNKRQINPDVLLGKVIGSEPIDMFKMTAALGKHIHKKT

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PEQL01000003
EMBL· GenBank· DDBJ
PJD97551.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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