A0A2H4NRK4 · A0A2H4NRK4_AMYSP

  • Protein
    DNA ligase
  • Gene
    ligA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

158950100150200250300350400450500550
TypeIDPosition(s)Description
Binding site51-55NAD+ (UniProtKB | ChEBI)
Binding site130NAD+ (UniProtKB | ChEBI)
Active site132N6-AMP-lysine intermediate
Binding site153NAD+ (UniProtKB | ChEBI)
Binding site187NAD+ (UniProtKB | ChEBI)
Binding site303NAD+ (UniProtKB | ChEBI)
Binding site327NAD+ (UniProtKB | ChEBI)
Binding site421Zn2+ (UniProtKB | ChEBI)
Binding site424Zn2+ (UniProtKB | ChEBI)
Binding site443Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Hca4
  • Taxonomic lineage
    Bacteria > Actinomycetota > Actinomycetes > Pseudonocardiales > Pseudonocardiaceae > Amycolatopsis

Accessions

  • Primary accession
    A0A2H4NRK4

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain22-457NAD-dependent DNA ligase N-terminal
Region555-589Disordered
Compositional bias573-589Polar residues

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    589
  • Mass (Da)
    61,877
  • Last updated
    2018-02-28 v1
  • Checksum
    A51EC15C415C856A
MTTELAPGTGAPVDPAKAKPFPSVAGYEKALAEIRAAAAAYYAGADVVMDDDTYDALLARAIATEAAHPAWKPADTPTEAVGAGVAPGTEIPHSTPMLGLDNVFDSESLRKWATRLERVLGHPVTAYTVEPKLDGLAVAARYSRGRLTLVLTRGTGTMGEDVTTRAVLAKGLPTRLSSPRTIEVRGEVFMTDEDFRTANELRTGHGEPAFAHPRSAAAGTLRAVDRAYSAPLSFTAYAVQGLADDGTHSEAMRQLEELGVATTSGGKPALKVCRSAEEIEAAVAEIRDLRGALGFGVDGAVIKADRPQDRAAAGASAKAPRWGVAVKFPADTRSTKLLAIDVQVGRTGVITPVATLEPVFIDGVKVVSATLHNFDDLQRRNVRVGDTVFVRRAGDVIPEITGAKLDERPADARPFTPPEVCPDCGSEIDRSQTRWRCTGGRTCGARKILAHYATREAMDIEGMGGKIIDLLVAEGLVADPADLYDLDVPGLAALDGLGEISGGKLVAAIAATKTRPLSSLLTGLGIRMAGRAVSRRLARHFGSMDALLAATPSSCRRSKASAGNAPRRSRASWPSWPGSSRSSRPAGSP

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias573-589Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MF990204
EMBL· GenBank· DDBJ
ATV95622.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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