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A0A2G5WW82 · A0A2G5WW82_9BACL

  • Protein
    3-isopropylmalate dehydrogenase
  • Gene
    leuB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mg2+ or Mn2+ ion per subunit.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site76-89NAD+ (UniProtKB | ChEBI)
Binding site96substrate
Binding site106substrate
Binding site134substrate
Site141Important for catalysis
Site187Important for catalysis
Binding site219Mg2+ (UniProtKB | ChEBI)
Binding site219substrate
Binding site243Mg2+ (UniProtKB | ChEBI)
Binding site247Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3-isopropylmalate dehydrogenase activity
Molecular Functionmagnesium ion binding
Molecular FunctionNAD binding
Biological ProcessL-leucine biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    3-isopropylmalate dehydrogenase
  • EC number
  • Alternative names
    • 3-IPM-DH
    • Beta-IPM dehydrogenase
      (IMDH
      )

Gene names

    • Name
      leuB
    • ORF names
      CSV78_04875

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • P16a
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Planococcaceae > Sporosarcina

Accessions

  • Primary accession
    A0A2G5WW82

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Homodimer.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-348Isopropylmalate dehydrogenase-like

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    366
  • Mass (Da)
    40,069
  • Last updated
    2018-01-31 v1
  • MD5 Checksum
    B720723C6150EF81957AEA2A15BBCE38
MEKKVAVLPGDGIGPEVVAEAVRVLEVIGRRFNHTFHTEYGLIGGAAIDEKNNPLPKETIELCESSDAILLGAVGGPKWDQNPSELRPEKGLLAIRKHFDLFANLRPVAAVPSLIQASPLKEEIVKEVDMMIVRELTGGLYFGKPSRHTDQSAVDTLVYTREEIERIVDKGFELARLRKGKVTSVDKANVLESSKLWRKIVEEKKAQYPDVEVEHQLVDSAAMKLITNPAYFDVIVTENMFGDILSDEASVITGSLGLLPSASVRSDGLGLYEPVHGSAPDVAGQGIANPAATILSVAMMLKYSFGLETEATAIEKAVNTVFEEGHFTADLAGKDQRSLTTKEWTDKIVDELDLQFVSNSIMYSYI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
PDYN01000003
EMBL· GenBank· DDBJ
PIC68127.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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