A0A2E5WMY1 · A0A2E5WMY1_UNCXX
- ProteinPhosphoribosylglycinamide formyltransferase
- GenepurN
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids922 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the transfer of a formyl group from 10-formyltetrahydrofolate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR) and tetrahydrofolate.
Catalytic activity
- (6R)-10-formyltetrahydrofolate + N1-(5-phospho-beta-D-ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H+ + N2-formyl-N1-(5-phospho-beta-D-ribosyl)glycinamide
Pathway
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N1-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Purine metabolism; IMP biosynthesis via de novo pathway; N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide from N1-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 725-727 | N1-(5-phospho-beta-D-ribosyl)glycinamide (UniProtKB | ChEBI) | ||||
Sequence: GSN | ||||||
Binding site | 782 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 807-810 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: MRIL | ||||||
Binding site | 824 | (6R)-10-formyltetrahydrofolate (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 826 | Proton donor | ||||
Sequence: H | ||||||
Site | 865 | Raises pKa of active site His | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | phosphoribosylamine-glycine ligase activity | |
Molecular Function | phosphoribosylformylglycinamidine cyclo-ligase activity | |
Molecular Function | phosphoribosylglycinamide formyltransferase activity | |
Biological Process | 'de novo' IMP biosynthetic process | |
Biological Process | adenine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Subcellular Location
UniProt Annotation
GO Annotation
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 109-313 | ATP-grasp | ||||
Sequence: LLKESNYDCYNPKFNMISGENDIINIIDKYDKFVIKLNGLAGGKGVFVQDDHFKTREEGIKIIKQKIKEESILIEEKLEGQEFSLFTLTDGYSSFHFPPVQDYKRAYDNDNGPNTGGMGSIMTDFDFLNEEDIVKCEEINNNIIRIIQQKYNEPYIGILYGSFMMTNSKQIKLIEYNCRFGDSEVFNLLNSLETDLSIIFKHMAE |
Sequence similarities
Belongs to the AIR synthase family.
Belongs to the GART family.
In the C-terminal section; belongs to the GART family.
In the N-terminal section; belongs to the GARS family.
In the central section; belongs to the AIR synthase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length922
- Mass (Da)104,878
- Last updated2018-01-31 v1
- Checksum62BFD649716EDC37