A0A2E0ZRQ3 · A0A2E0ZRQ3_9ACTN
- ProteinPhosphoenolpyruvate carboxykinase [GTP]
- GenepckG
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids597 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Catalytic activity
- GTP + oxaloacetate = CO2 + GDP + phosphoenolpyruvate
Cofactor
Note: Binds 1 Mn2+ ion per subunit.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 72 | substrate | ||||
Sequence: R | ||||||
Binding site | 211-213 | substrate | ||||
Sequence: YGG | ||||||
Binding site | 220 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 240 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 262 | substrate | ||||
Sequence: S | ||||||
Binding site | 263-268 | GTP (UniProtKB | ChEBI) | ||||
Sequence: ACGKTN | ||||||
Active site | 264 | |||||
Sequence: C | ||||||
Binding site | 287 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 377-379 | substrate | ||||
Sequence: NAR | ||||||
Binding site | 379 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 410 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 505-508 | GTP (UniProtKB | ChEBI) | ||||
Sequence: FGEN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | GTP binding | |
Molecular Function | kinase activity | |
Molecular Function | manganese ion binding | |
Molecular Function | phosphoenolpyruvate carboxykinase (GTP) activity | |
Biological Process | cellular response to glucose stimulus | |
Biological Process | gluconeogenesis | |
Biological Process | glycerol biosynthetic process from pyruvate | |
Biological Process | propionate catabolic process | |
Biological Process | response to lipid | |
Biological Process | response to starvation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoenolpyruvate carboxykinase [GTP]
- EC number
- Short namesPEP carboxykinase ; PEPCK
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageBacteria > Actinomycetota > Acidimicrobiia > Acidimicrobiales > Acidimicrobiaceae
Accessions
- Primary accessionA0A2E0ZRQ3
Proteomes
Subcellular Location
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 12-232 | Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal | ||||
Sequence: EWVDHWTEVLGPDTVHWCDGTEAEYDALAAGMVASGTFRRLNDDLRPNSYLALSDPEDVARVEDRTFIATKDPLDAGPTNNWRDPYELKAEMTGHFQGAMAGRTMYVVPFSMGPLGSPIAHIGIQLTDSPYVVAGMRXMTRMGRPVLXVLGQGDFVPCVHSVGAPLVDGAEDVSWPCNPDHRYIAHFPKTREIWSYGSGYGGNALLGKKCFALRIASTMAH | ||||||
Domain | 236-594 | Phosphoenolpyruvate carboxykinase C-terminal P-loop | ||||
Sequence: WLAEHMLXLAVTPPGGERRYVAAAFPSACGKTNMAMMVPTLPGWTVETIGDDIAWMKFXDDGRLYAINPEAGFFGVAPGTSEASNHNAMATLHANAIFTNVARTDDGDVWWEGMTDEPPEHLTDWRGDPWTPDNGSKAAHPNARFTVSAAQCPSIAPEWEDPAGVPISAILFGGRRATNVPLVTEARDWXHGVFLGSIMSSEKTAAASGQTGTVRFDPFAMLPFTGYNVGDYFAHWLEIGARADADRLPKLFWVNWFRRDGDRSFLWPGFGENCRVLDWVLRRVEGTGDAVETPIGLVPTIDAIDRTGIDVDDAAMEQLLDVNQVAWEAELAMIEEHYAAVGERLPVELRRQLDDLRDR | ||||||
Region | 344-375 | Disordered | ||||
Sequence: VWWEGMTDEPPEHLTDWRGDPWTPDNGSKAAH |
Sequence similarities
Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length597
- Mass (Da)65,566
- Last updated2018-04-25 v1
- Checksum192775B80237938D