A0A2D7I2T4 · A0A2D7I2T4_UNCDE

Function

function

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site268-269ATP (UniProtKB | ChEBI)
Binding site350ATP (UniProtKB | ChEBI)
Binding site372ATP (UniProtKB | ChEBI)
Binding site381ATP (UniProtKB | ChEBI)
Active site535Cysteine persulfide intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular FunctionATP binding
Molecular FunctionCCA tRNA nucleotidyltransferase activity
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uracil-4 sulfurtransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiamine diphosphate biosynthetic process
Biological Processthiazole biosynthetic process
Biological ProcesstRNA 4-thiouridine biosynthesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA sulfurtransferase
  • EC number
  • Alternative names
    • Sulfur carrier protein ThiS sulfurtransferase
    • Thiamine biosynthesis protein ThiI
    • tRNA 4-thiouridine synthase

Gene names

    • Name
      thiI
    • ORF names
      CL917_13620

Organism names

Accessions

  • Primary accession
    A0A2D7I2T4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Keywords

Family & Domains

Features

Showing features for region, compositional bias, domain.

TypeIDPosition(s)Description
Region1-88Disordered
Compositional bias14-37Polar residues
Domain145-250THUMP
Domain487-574Rhodanese

Sequence similarities

Belongs to the ThiI family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    577
  • Mass (Da)
    64,198
  • Last updated
    2018-04-25 v1
  • Checksum
    B8EADE0CAC9EBDF9
MGPWVDHPPNPLKKSECSGSQSDPGGPLCTTITGRLSENHHAELRSVPLQGYVMKDTAPGQKRVKNAETGPSSEESPRSPSPTGKERIIVRFSGDITTKSAPTRKRFLTRMSRNIKNALSTASIENRVSRTHTRIFVDIDSDRIDAALQLVSHIFGVQSASRVEERKWNDADDIVTAGREIFLESVRNKVFAVRAKRVGNRNAIPIDSTYVERELGSELLKSARKVSLKNPEAIAAIEILADQAHFFSHTVQSPGGLPLGCEGRAVALVSGGFDSPVAAWLLNKRGVELDYVFCNLGGRTHQLETLQVMKVMADQWSYGTRPHFHAIEFEEVTRDLQAGVTTRYWQIILKRLMMRAAEVIARERDASAIVTGEAVGQVSSQTLPNLAVISQATSLPILRPLIGYNKDEIIALARKIGTHDLSSKVGEYCALVPSRPATRATQKRIEAEEMALDPKILDRAIAERSIFDLRALDLESLDRPEIQTEKINETDTVIDLRSKNAYENWHYPKALFLEFQDAMKAYPHMDHEKRYVLYCEFGLKSGHLAELMRREGLNASHFVGGLKAITQYAKQTGAEEP

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias14-37Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
NZHD01000089
EMBL· GenBank· DDBJ
MAI79979.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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